Anti-Histone H2A (symmetric di methyl R3) 抗体 (ab22397)

製品の概要

  • 製品名Anti-Histone H2A (symmetric di methyl R3) antibody
    Histone H2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H2A (symmetric di methyl R3)
  • アプリケーション適用あり: IHC-P, ICC/IF, ELISAmore details
  • 種交差性

    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H2A, symmetric di methylated at R3.

    (Peptide available as ab22399.)

  • ポジティブ・コントロール
    • IHC-P: Human normal kidney FFPE tissue sections. ICC/IF: MCF-7 cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab22397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

This antibody gave a positive result in ELISA against the immunizing peptide (ab22399). It gave a negative result in ELISA against the non-modified equivalent peptide (ab13186). This indicates that it is specific for the modified peptide. See figure below. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 機能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性Belongs to the histone H2A family.
  • 翻訳後修飾The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FO108 antibody
    • H2A antibody
    • H2A1B_HUMAN antibody
    • H4 histone family 2 antibody
    • H4 histone family member N antibody
    • H4F2 antibody
    • H4FN antibody
    • HIST1H2AE antibody
    • HistH2A antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    see all

Anti-Histone H2A (symmetric di methyl R3) antibody 画像

  • IHC image of ab22397 staining Histone H2A (symmetric di methyl R3) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22397, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • This antibody gave a positive result in ELISA against the immunizing peptide ab22399 (blue line). Histone H2A (unmodified) peptide ab13186 (pink line) gave a negative result, This indicates that it is specific for the Histone H2A (symmetric di methyl R3) peptide.
  • ICC/IF image of ab22397 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab22397, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.  However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.

Anti-Histone H2A (symmetric di methyl R3) antibody (ab22397) 使用論文

This product has been referenced in:
  • Deng X  et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene N/A:N/A (2016). WB, ChIP ; Human . Read more (PubMed: 27546619) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. For pairing with ab22397, I would recommend against using ab6341 (same clone as ab22397, but unconjugated) or ab6344 (recognizes the same epitope as the ab22397 clone). You may wish to take a look at ab13417 or ab282...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"