Anti-Histone H2A (acetyl K5) 抗体 [EP856Y] (ab45152)

製品の概要

  • 製品名Anti-Histone H2A (acetyl K5) antibody [EP856Y]
    Histone H2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP856Y] to Histone H2A (acetyl K5)
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Caenorhabditis elegans
  • 免疫原

    Synthetic peptide corresponding to residues near the N-terminus of human Histone H2A.

  • ポジティブ・コントロール
    • Human breast carcinoma tissue and C6 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名EP856Y
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100000. Predicted molecular weight: 14 kDa.
IHC-P 1/250 - 1/2000.
ICC/IF 1/200 - 1/1000.
IP 1/20.

ターゲット情報

  • 機能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性Belongs to the histone H2A family.
  • 翻訳後修飾The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • H2A antibody
    • H2A1B_HUMAN antibody
    • H2AFM antibody
    • HIST1H2A antibody
    • HIST1H2AE antibody
    • Histone H2A type 1-B/E antibody
    • Histone H2A.2 antibody
    • Histone H2A/a antibody
    • Histone H2A/m antibody
    see all

Anti-Histone H2A (acetyl K5) antibody [EP856Y] 画像

  • ab45152 staining Histone H2A (acetyl K5) in C6 (rat glioma) cells, treated and untreated with TSA,500ng/ml for 4 hours by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

     

  • ab45152 staining Histone H2A (acetyl K5) in rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • ab45152 immunoprecipitating Histone H2A. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: Mouse brain whole cell lysate
    Lane 2: Mouse brain whole cell lysate 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45152 in Mouse brain whole cell lysate 

     

  • Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/1000 dilution (unpurified) + Mouse cortex whole tissue lysate at 20 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC - Wholemount of Caenorhabditis elegans embryo labelling Histone H2A (acetyl K5) with unpurified ab45152. Sample was incubated with primary antibody (1/500 in 3% BSA + 0.1% Triton in TBS buffer) for 12 hours at 4°C. An Alexa Fluor® 546-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Top - Telomere (green), Middle - Histone H2A (acetyl K5) (red), Bottom - merge.

    See Abreview

  • ab45152 staining Histone H2A (acetyl K5) in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/100000 dilution (unpurified)

    Lane 1 : C6 cell lysate
    Lane 2 : C6 + TSA membrane


    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
  • ab45152 staining Histone H2A (acetyl K5) in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

     

Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) 使用論文

This product has been referenced in:
  • Yang X  et al. Histone acetyltransferase 1 promotes homologous recombination in DNA repair by facilitating histone turnover. J Biol Chem 288:18271-82 (2013). Human . Read more (PubMed: 23653357) »

See 1 Publication for this product

Product Wall

Application IHC - Wholemount
Sample Caenorhabditis elegans Embryo (C. elegans embryo)
Specification C. elegans embryo
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投稿 Dec 18 2013

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - nuclear (Fruit fly embryo)
Specification Fruit fly embryo
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Dec 18 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH=6.0
Sample Mouse Tissue sections (Mouse cortex)
Specification Mouse cortex
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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投稿 Dec 18 2013

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15%)
Sample Mouse Tissue lysate - whole (Mouse cortex)
Specification Mouse cortex
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Dec 18 2013

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Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 15 µg
Specification Liver
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 May 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HuH-7 cell line)
Loading amount 15 µg
Specification HuH-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 May 07 2012

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (primary neurons)
Specification primary neurons
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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投稿 Apr 23 2011

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Application Western blot
Sample Mouse Cell lysate - nuclear (primary Neurons)
Loading amount 40 µg
Specification primary Neurons
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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投稿 Apr 18 2011

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Application Western blot
Sample Human Cell lysate - nuclear (Keratinocytes)
Loading amount 20 µg
Specification Keratinocytes
Gel Running Conditions Reduced Denaturing (18)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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投稿 Apr 18 2011

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Application Western blot
Sample Human Cell lysate - nuclear (keratinocytes)
Loading amount 20 µg
Specification keratinocytes
Gel Running Conditions Reduced Denaturing (20%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jan 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"