製品の概要

  • 製品名Anti-Histone H1x antibody
    Histone H1x 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H1x
  • 特異性ab17729 recognises Histone H1x variant in HeLa nuclear and whole cell extracts (see figures below).
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Histone H1x aa 150 to the C-terminus (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q92522
    (Peptide available as ab18053)

  • ポジティブ・コントロール
    • HeLa whole cell lysate and HeLa nuclear lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

Anti-Histone H1x antibody 画像

  • All lanes : Anti-Histone H1x antibody (ab17729) at 0.5 µg/ml

    Lane 1 : HeLa whole cell extract
    Lane 2 : HeLa whole cell extract with Human Histone H1x peptide (ab18053) at 1 µg/ml
    Lane 3 : HeLa nuclear extract


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    ab17729 recognises histone H1.X in HeLa whole cell (lane1) and nuclear extracts (lane3) at 35 kDa, which is efficiently blocked using the immunizing peptide ab18053 (lane2). ab17729 recognises bands at 20 kDa, which is attributed to minor cross-reactivity and may be improved using different blocking conditions.
  • ICC/IF image of ab17729 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab17729, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas

    ab17729 staining histone H1.X in male cerebellum, showing a distinct and strong nuclear staining pattern at cells in granular and molecular layers. Paraffin embedded human skin tissue was incubated with ab17729 (1/750 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab17729 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

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Anti-Histone H1x antibody (ab17729) 使用論文

This product has been referenced in:
  • Crawford NP  et al. The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor. J Biol Chem 284:28660-73 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19710015) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"