This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Histone H1.4 (tri methyl K25, phospho S26) 抗体 (ab33231)

製品の概要

  • 製品名
    Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody
    Histone H1.4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H1.4 (tri methyl K25, phospho S26)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 由来種
    Rabbit
  • 種交差性

    交差が予測される動物種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1.4, tri methylated at K25 and phosphorylated at S26.

    (Peptide available as ab35913.)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

アプリケーション Abreviews 特記事項
ELISA
  • 追加情報
    This antibody gave a positive result in ELISA against the immunizing peptide.
    For the following peptides it gave following results:
    the non-modified equivalent peptide (yellow line, ab35918) - weak result,
    H1.4 phospho S26 peptide (pink line, ab35917) - weak result.
    See figure below.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
    • 配列類似性
      Belongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.
    • 翻訳後修飾
      Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
    • 細胞内局在
      Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • H1 histone family member 4 antibody
      • H1.4 antibody
      • H14_HUMAN antibody
      • H1E antibody
      • H1F4 antibody
      • Hist1h1e antibody
      • Histone 1 H1e antibody
      • Histone cluster 1 H1e antibody
      • Histone H1 antibody
      • Histone H1.4 antibody
      • Histone H1B antibody
      • MGC116819 antibody
      see all

    画像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • This antibody gave a positive result in ELISA against the immunizing peptide (blue line, ab35913).
      For the following peptides it gave following results:
      the non-modified equivalent peptide (yellow line, ab35918) - weak result,
      H1.4 phospho S26 peptide (pink line, ab35917) - weak result.

    参考文献

    ab33231 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Purified protein (breast cancer cell line T47D)
    Loading amount
    5 µg
    Specification
    breast cancer cell line T47D
    Treatment
    50ng/ml colcemid for 16h
    Gel Running Conditions
    Non-reduced Denaturing (10 % SDS-PAGE)
    Blocking step
    Odissey blocking solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Dr. Albert Jordan

    Verified customer

    投稿 May 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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