製品の概要

  • 製品名Anti-Histone H1.4 (phospho T18) antibody
    Histone H1.4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H1.4 (phospho T18)
  • アプリケーション適用あり: WB, ICC/IF, ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1, phosphorylated at T18.

    (Peptide available as ab10138.)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 32 kDa.
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

ターゲット情報

  • 機能Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • 配列類似性Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 翻訳後修飾Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • H1 histone family member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1 H1e antibody
    • Histone cluster 1 H1e antibody
    • Histone H1 antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    see all

Anti-Histone H1.4 (phospho T18) antibody 画像

  • This image is courtesy of Alejandro Contreras, Baylor College of Medicine

    Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).

    Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.

    Lanes 1-2    :  wt Histone H1
    Lanes 3-4    :  T146A, T154A, S172A, S187A (wt site at T18)
    Lanes 5-6    :  T18A, T154A, S172A, S187A (wt site at T146)
    Lanes 7-8    :  T18A, T146A, S172A, S187A (wt site at T154)
    Lanes 9-10  :  T18A, T146A, T154A, S187A (wt site at S172)
    Lanes 11-12: 

  • Rabbit polyclonal to Histone H1 (phospho T18) (1/25).

    Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab3595 (green). The DNA stained with DAPI is shown in red. (100x magnification).

Anti-Histone H1.4 (phospho T18) antibody (ab3595) 使用論文

This product has been referenced in:
  • Chen Y  et al. Quantitative Mass Spectrometry Reveals that Intact Histone H1 Phosphorylations are Variant Specific and Exhibit Single Molecule Hierarchical Dependence. Mol Cell Proteomics N/A:N/A (2015). Read more (PubMed: 26209608) »

See 1 Publication for this product

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Thank you for your email and I'm sorry to hear that you are experiencing difficulty with ab3595. It would be very helpful if you could provide me with some additional details regarding your protocol. This will help us to investigate this matter as quic...

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Thank you for your e-mail. We are very sorry to hear that you are having problem with this antibody. We do not have problem with this antibody and this is the first complaint we have received so far. Certainly, we can send you a new vial as a repl...

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