製品の概要

  • 製品名Anti-Histone H1.4 antibody
    Histone H1.4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H1.4
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 1-30 of Human Histone H1.4 (NP_005312.1).

  • ポジティブ・コントロール
    • MDA-MB435 cell line lysates. IHC-P: Human normal colon FFPE tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab116393 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 22 kDa.

ターゲット情報

  • 機能Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • 配列類似性Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 翻訳後修飾Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ221C16.5 antibody
    • H1 histone family, member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1, H1e antibody
    • Histone cluster 1, H1e antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    • OTTHUMP00000016139 antibody
    see all

Anti-Histone H1.4 antibody 画像

  • IHC image of ab116393 staining Histone H1.4 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116393, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Histone H1.4 antibody (ab116393) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

    Predicted band size : 22 kDa

Anti-Histone H1.4 antibody (ab116393) 使用論文

ab116393 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"