製品の概要

  • 製品名Anti-Histone H1.4 antibody
    Histone H1.4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histone H1.4
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, African Green Monkey
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MSETAPAAPA APAPAEKTPV KKKARKSAGA AKRKASGPPV SELITKAVAA) of Human Histone H1.4 (NP_005312).

  • ポジティブ・コントロール
    • ACHN cell lysate IF/ICC: SKNSH cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab105522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.Can be blocked with Histone H1.4 peptide (ab130902). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
  • 配列類似性Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 翻訳後修飾Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ221C16.5 antibody
    • H1 histone family, member 4 antibody
    • H1.4 antibody
    • H14_HUMAN antibody
    • H1E antibody
    • H1F4 antibody
    • Hist1h1e antibody
    • Histone 1, H1e antibody
    • Histone cluster 1, H1e antibody
    • Histone H1.4 antibody
    • Histone H1B antibody
    • MGC116819 antibody
    • OTTHUMP00000016139 antibody
    see all

Anti-Histone H1.4 antibody 画像

  • ICC/IF image of ab105522 stained SKNSH cells. The cells were4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-Histone H1.4 antibody (ab105522) at 1 µg/ml + ACHN cell lysate at 10 µg

    Predicted band size : 22 kDa
    Gel concentration: 12%

Anti-Histone H1.4 antibody (ab105522) 使用論文

ab105522 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab105522.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"