Anti-Histone H1.2 抗体 (ab17677)
Key features and details
- Rabbit polyclonal to Histone H1.2
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H1.2 antibody
Histone H1.2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H1.2 -
由来種
Rabbit -
特異性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, IP, WBmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in Human H1.2 Recombinant Protein. ICC/IF: HeLa cell line
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17677の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (3) |
Use a concentration of 1 µg/ml.
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IP |
Use a concentration of 5 µg/ml.
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WB | (2) |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IP
Use a concentration of 5 µg/ml. |
WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
ターゲット情報
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機能
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
配列類似性
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 3006 Human
- Entrez Gene: 50708 Mouse
- Omim: 142710 Human
- SwissProt: P16403 Human
- SwissProt: P15864 Mouse
- Unigene: 7644 Human
- Unigene: 193539 Mouse
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別名
- H1 histone family member 2 antibody
- H1.a antibody
- H12_HUMAN antibody
see all
画像
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All lanes : Anti-Histone H1.2 antibody (ab17677) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HIST1H1C knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Histone H1.2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab17677 was shown to bind specifically to Histone H1.2. A band was observed at 32 kDa in wild-type HeLa cell lysates with no signal observed at this size in HIST1H1C knockout cell line ab261794 (knockout cell lysate ab257218). To generate this image, wild-type and HIST1H1C knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-Histone H1.2 antibody (ab17677) at 1/1000 dilution
Lane 1 : Recombinant histone H1
Lane 2 : Recombinant histone H1.1
Lane 3 : Recombinant histone H1.2
Lane 4 : Recombinant histone H1.3
Lane 5 : Recombinant histone H1.4
Lane 6 : Recombinant histone H1.5
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Histone H1.2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Histone H1.2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17677.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 35kDa; Histone H1.2
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ab17677 staining Histone H1.2 in HeLa cells. The cells were fixed with 100% methanol (5 min) at room temperature, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tritonn for 1h. The cells were then incubated with the antibody ab16766 at 0.1µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
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All lanes : Anti-Histone H1.2 antibody (ab17677) at 1 µg/ml
Lane 1 : Histone H1 Recombinant Protein
Lane 2 : Histone H1.2 (Human) - Recombinant Protein
Lane 3 : Histone H1 Recombinant Protein with Human Histone H1.2 peptide (ab18500) at 1 µg/ml
Lane 4 : Histone H1.2 (Human) - Recombinant Protein with Human Histone H1.2 peptide (ab18500) at 1 µg/ml
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 12 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (32)
ab17677 は 32 報の論文で使用されています。
- Höllmüller E et al. Site-specific ubiquitylation acts as a regulator of linker histone H1. Nat Commun 12:3497 (2021). PubMed: 34108453
- Olins AL et al. Hyperosmotic stress: in situ chromatin phase separation. Nucleus 11:1-18 (2020). PubMed: 31924112
- Mandemaker IK et al. Histone H1 eviction by the histone chaperone SET reduces cell survival following DNA damage. J Cell Sci 133:N/A (2020). PubMed: 32184266
- Chen J et al. A Method for Extracting the Nuclear Scaffold from the Chromatin Network. Bio Protoc 8:e2821 (2018). PubMed: 34286031
- Bernstein DI et al. Genetic variants with gene regulatory effects are associated with diisocyanate-induced asthma. J Allergy Clin Immunol 142:959-969 (2018). PubMed: 29969634