製品の概要

  • 製品名Anti-Histone H1.02 antibody - ChIP Grade
  • 製品の詳細
    Sheep monoclonal to Histone H1.02 - ChIP Grade
  • アプリケーション適用あり: WB, ChIP, IHC-Pmore details
  • 種交差性
    交差種: Chicken
  • 免疫原

    N-terminal peptide (APVAAPAVSAPGAK-C) of chicken linker histone H1.02.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.

Anti-Histone H1.02 antibody - ChIP Grade 画像

  • Competition Western Blot Analysis. The recombinant linker histone was separated on a 15% SDS PAGE slab gel together with a track loaded with total chicken histone as marker and transferred to nitrocellulose membranes. These were then cut into strips of equal width and each strip developed with the antibody which had been pre-incubated with one of the recombinant linker histone at four increasing concentrations (two are shown). The panels show (from left to right): the transfer of the recombinant protein slab gel; the transfer of a total chicken histone marker, M; the histone marker strip blotted with the antibody under test, M(WB), showing that there is no recognition of the core histones, nor H5, with the antibody; six strips developed with the test antibody (at 0.1 mg/ml) pre-incubated with the six recombinant histones (01 to R) at the indicated concentrations. The Control lanes represent blots of a seventh strip developed with the test antibody without any competitor. The red boxes show tracks in which signal has been competed away.

Anti-Histone H1.02 antibody - ChIP Grade (ab100945) 使用論文

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