製品の概要

  • 製品名
    Anti-Histone H1.0 antibody [34]
    Histone H1.0 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [34] to Histone H1.0
  • アプリケーション
    適用あり: WB, Flow Cyt, ICC/IF, ICC, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Xenopus laevis, Vertebrata非交差種: Bird
  • 免疫原

    Ox Liver Histone H1.0.

  • エピトープ
    This antibody recognises an epitope within aa20-30. Proline 26 is not essential for recognition by the antibody. See Gorka et al. 1998 for more information.
  • ポジティブ・コントロール
    • Murine erythroleukemia (MEL) cells were used in part of the original characterisation of this antibody. Purified histones can also be used as a positive control. This antibody also gave a positive signal in human breast adenocarcinoma tissue sections. In Flow Cytometry, this antibody gave a positive signal in HeLa cells. ICC/IF - HeLa cells
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 0.4M arginine
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    34
  • ミエローマ
    NS1/1-Ag4-1
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 20 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 10 µg/ml.

For better results, cells should be fixed with methanol.

ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
EMSA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
  • 配列類似性
    Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 翻訳後修飾
    Phosphorylated on Ser-17 in RNA edited version.
  • 細胞内局在
    Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • H1 histone family member 0 antibody
    • H1(0) antibody
    • H10 antibody
    • H10_HUMAN antibody
    • h1f0 antibody
    • H1FV antibody
    • Histone H1'' antibody
    • Histone H1(0) antibody
    • Histone H1.0 antibody
    • Histone H10 antibody
    • Histone H5 antibody
    • MGC5241 antibody
    • N-terminally processed antibody
    see all

Anti-Histone H1.0 antibody [34] 画像

  • ab11079 staining Histone H1.0 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab11079 at 10µg/ml and ab6046 (anti beta Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Rabbit secondary (ab150084) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.



  • Predicted band size : 20 kDa

    Western blot using ab11079 on HeLa whole cell extract (30 µg/lane).

    Exposure 5 sec using ECL.
    12.5% SDS-PAGE gel.

  • Overlay histogram showing HeLa cells stained with ab11079 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • IHC image of ab11079 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11079, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


  • Predicted band size : 20 kDa

    Dr Albert Jordan, Center for Genomic Regulation (CRG), Spain.

    Mouse monoclonal to Histone H1.0 against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure.

    Dilution 1/1000.

    Recombinant H1 isoforms courtesy of Dr Nicole Happel.

    (see attached review)

Anti-Histone H1.0 antibody [34] (ab11079) 使用論文

This product has been referenced in:
  • Mayor R  et al. Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions. J Biol Chem 290:7474-91 (2015). WB ; Human . Read more (PubMed: 25645921) »
  • Büchner A  et al. Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity. Neuromolecular Med 17:12-23 (2015). Read more (PubMed: 25391294) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
2 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC 5VA - Lung Fibroblasts)
Loading amount
15 µg
Specification
MRC 5VA - Lung Fibroblasts
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Mar 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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