製品の概要

  • 製品名Anti-Histone H1 antibody [AE-4]
    Histone H1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AE-4] to Histone H1
  • 特異性Histone H1 itself is a highly conserved protein and is practically identical in sequence across most species. Given this, species cross-reactivity may be expected. In blots of rat brain homogenate a duplet is observed at 27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
  • アプリケーション適用あり: IHC-Fr, IHC-P, ELISA, WB, ICC, ICC/IF, Electron Microscopymore details
  • 種交差性
    交差種: Human
  • 免疫原

    Nuclei from myeloid leukamia biopsy cells.

  • ポジティブ・コントロール
    • IHC-P: Human normal colon FFPE tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB 1/100.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration. PubMed: 21063722

ターゲット情報

  • 機能May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
  • 組織特異性Oocyte-specific.
  • 配列類似性Belongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • 細胞内局在Cytoplasm. Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ221C16.2 antibody
    • dJ221C16.5 antibody
    • DmeH1 antibody
    • H1 0 histone antibody
    • H1 antibody
    • H1 histone family member 0 antibody
    • H1 histone family member 1 antibody
    • H1 histone family member 3 antibody
    • H1 histone family member 5 antibody
    • H1 histone family member N testis specific antibody
    • H1 histone family member O oocyte specific antibody
    • H1 histone family member X antibody
    • H1(0) antibody
    • H1.1 antibody
    • H1.2 antibody
    • H1.3 antibody
    • H1.4 antibody
    • H1.5 antibody
    • H10 antibody
    • H1A antibody
    • H1f0 antibody
    • H1F0 protein antibody
    • H1F1 antibody
    • H1F2 antibody
    • H1F3 antibody
    • H1F4 antibody
    • H1F5 antibody
    • H1FNT antibody
    • H1FOO antibody
    • H1FOO protein antibody
    • H1FOO_HUMAN antibody
    • H1FT antibody
    • H1FV antibody
    • H1FX antibody
    • H1oo antibody
    • H1t antibody
    • H1T2 antibody
    • H1X antibody
    • HANP1 antibody
    • His1 antibody
    • HisC antibody
    • HIST1 antibody
    • HIST1H1A antibody
    • HIST1H1B antibody
    • HIST1H1C antibody
    • HIST1H1D antibody
    • HIST1H1E antibody
    • HIST1H1T antibody
    • Histone 1 H1a antibody
    • Histone 1 H1b antibody
    • Histone 1 H1c antibody
    • Histone 1 H1d antibody
    • Histone 1 H1e antibody
    • Histone 1 H1t antibody
    • Histone antibody
    • Histone H1' antibody
    • Histone H1.0 antibody
    • Histone H1.1 antibody
    • Histone H1.2 antibody
    • Histone H1.3 antibody
    • Histone H1.4 antibody
    • Histone H1.5 antibody
    • Histone H1oo antibody
    • Histone H1x antibody
    • MGC116819 antibody
    • MGC126630 antibody
    • MGC126632 antibody
    • MGC126642 antibody
    • MGC138176 antibody
    • MGC138345 antibody
    • MGC15959 antibody
    • MGC3992 antibody
    • MGC50807 antibody
    • MGC5241 antibody
    • MGC8350 antibody
    • Oocyte specific histone H1 antibody
    • Oocyte specific linker histone H1 antibody
    • Oocyte-specific histone H1 antibody
    • Oocyte-specific linker histone H1 antibody
    • osH1 antibody
    • Testicular H1 histone antibody
    see all

Anti-Histone H1 antibody [AE-4] 画像

  • IHC image of ab71594 staining Histone H1 in Human normal colon formalin fixed paraffin embedded tissue* sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71594, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab71594 staining Histone H1 in HeLa cells by Immunocytochemistry/ Electron Microscopy.
    Cells were frozen under high pressure. Samples were incubated with ab71594 at 5µg/ml in 1% serum, 1% BSA, PBS. The secondary used was a donkey anti-mouse conjugated to 10nM gold, 1/30 dilution.

Anti-Histone H1 antibody [AE-4] (ab71594) 使用論文

This product has been referenced in:
  • Kagiwada S  et al. Replication-coupled passive DNA demethylation for the erasure of genome imprints in mice. EMBO J 32:340-53 (2013). Read more (PubMed: 23241950) »
  • Singh SK  et al. GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy. Nat Commun 4:1830 (2013). Human . Read more (PubMed: 23652018) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Lung)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 20 µg
Specification Lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Herr Dr. Paul Vesely

Verified customer

投稿 Feb 23 2016

Thank you for contacting us.

ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

ab83058; the sequence used is from P07305 http://...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (HeLa cells)
Specification HeLa cells
Fixative high-pressure freezing
Permeabilization No
Username

Dr. Margarita Sobol

Verified customer

投稿 Jan 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"