製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Histone H1
  • 特異性
    Detects total Histone H1 levels.
  • アプリケーション
    適用あり: IHC-P, WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human Histone H1 around the phosphorylation site of threonine 17 (E-K-TP-P-V).

  • ポジティブ・コントロール
    • IHC-P: Human normal colon FFPE tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

画像

  • All lanes : Anti-Histone H1 antibody (ab61177) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with synthesized peptide


    Predicted band size : 36 kDa
  • IHC image of ab61177 staining Histone H1 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61177, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab61177 at 1/500 - 1/1000 dilution staining Histone H1 in HuvEc cells, treated with serum (20%, 15mins) by Immunofluorescence.

参考文献

This product has been referenced in:
  • Jhunjhunwala S  et al. Neutrophil Responses to Sterile Implant Materials. PLoS One 10:e0137550 (2015). Mouse . Read more (PubMed: 26355958) »
  • Xiong W  et al. Estradiol promotes cells invasion by activating ß-catenin signaling pathway in endometriosis. Reproduction 150:507-16 (2015). WB ; Human . Read more (PubMed: 26432349) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Human Cell lysate - nuclear (huh7 liver)
Specification
huh7 liver
Treatment
infected with flavivirus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 12 2014

Thank you for your enquiry.

Based on the immunogen sequence, ab61177 should recognize
Histone H1.5 (http://www.uniprot.org/uniprot/P16401),
Histone H1.3 (http://www.uniprot.org/uniprot/P16402), and
Histone H1.4 (http://www.uni...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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