Customer reviews and FAQs

製品の概要

  • 製品名Histone Extraction Kit
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • 全工程の試験時間
    1h 00m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Mammal
  • 製品の概要

    ab113476 is suitable for a quick preparation of total histone extracts from mammalian cells and tissue samples. The minimal amount of starting materials can be as low as 105 cells or 1 mg of tissue. For the best results, the cell number should be greater than 106 cells or the tissue amount should be greater than 10 mg. A total of 100 standard extractions (use 107 cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 107 cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.

  • 特記事項

    Shipped at +4°C. Store refrigerated at +4°C before preparation.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab113476 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration. Total histone protein yield = 0.4mg/107 or 100mg tissue (yield may vary depending on cell or tissue type).

    Histone Extraction Kit 画像

    • SDS-PAGE Analysis of samples prepared using Histone extraction Kit
      SDS-PAGE Analysis of samples prepared using Histone extraction Kit
    • Functional Studies - Histone Extraction Kit
      Functional Studies - Histone Extraction Kit

      Histones were purified from mouse liver cells using ab113476. Each lane was loaded with 15μg of purified histone.

      Image courtesy of Kevin Dalgaard, MPI for Immunobiology & Epigenetics, Freiburg, DE

    • Functional Studies - Histone Extraction Kit (ab113476)
      Functional Studies - Histone Extraction Kit (ab113476)
      Histone extracts were prepared from MCF-7 cells using the ab113476 and acetyl histone H3-K9 was quantified.

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    Histone Extraction Kit (ab113476) 使用論文

    This product has been referenced in:
    • Alzrigat M  et al. EZH2 inhibition in multiple myeloma downregulates myeloma associated oncogenes and upregulates microRNAs with potential tumor suppressor functions. Oncotarget 8:10213-10224 (2017). WB ; Human . Read more (PubMed: 28052011) »
    • Gursoy-Yuzugullu O  et al. Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity. Oncotarget 8:24518-24532 (2017). Read more (PubMed: 28445939) »

    See all 13 Publications for this product

    ab113476 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab113476 の abreview を投稿する ab113476 に関する問い合わせをする

    27 Abreviews or Q&A

    10
    Votes: Highest First

    Please provide centrifugal force values for the RPM specifications in the the protocol. For example, 10k RPM = ? x g.
    Does the purified product need to be de-salted before running on a gel?

    The equivalent G Force values are:

    1000 RPM = 110G
    3000 RPM = 1000G
    10,000 RPM = 8000G
    12,000 RPM = 11,000G

    No special preparation such as desalting needs to be done before loading the sample into the gel.

    Dear technical support team: Why is so important to use this kit to isolation histone instead to use RIPA buffer? what is the difference?

    RIPA buffer can be used for extracting nuclear proteins but ab113476 is specifically for histone proteins. In general, the isolated histone protein concentration is much higher with use of ab113476than with RIPA buffer.

    Histone Extraction from a non-model insect

     Good Good 4/5 (Ease of Use)
    Abreviews
    The EpiSeeker Histone Extraction kit was used to isolate histone proteins for use with the EpiSeeker Histone H3 Total Acetylation Detection Fast Kit. Histones were extracted pupae of the flesh fly, Sarcophaga bullata, which is a non-model insect.

    The kit was easy and fast to use. It was easy to scale the reaction size to the amount of tissue.
    Username

    Dr. Julie Reynolds

    Verified customer

    投稿 Aug 21 2014

    Histone Extraction from a non-model insect

     Good Excellent 5/5 (Ease of Use)
    Abreviews
    This kit was used to isolate histones from pupae of the fleshy fly, Sarcophaga bullata, a non-model insect. A single pupa, weighing approximately 100 mg, was used in each sample. Each extraction yielded 14-19 ug/ul of histone protein.
    The kit was easy to use and simple to scale to the size of my insects. The extracted histones worked well with the EipSeeker Histone H3 Total Acetylation Detection Fast Kit.
    Username

    Abcam user community

    Verified customer

    投稿 Aug 20 2014

    Does this kit contain HDAC inhibitors?

    ab113476 does not contain any HDAC inhibitors.

    I am interested in H1 histone extraction from human blood and skin and had problems with an in-house acid extraction protocol. I wonder how the EpiSeeker histone extraction kit performs on H1. Would it be able to extract H1 quantitatively from these ti...

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    I can confirm that ab113476: EpiSeeker Histone Extraction Kit would be suitable to use for human blood and skin and would extract total histone proteins, including H1, that would be quantifiable.

    If I stored the cell pellets at -80oC, will the sample still be suitable for extracting nuclear protein and histone protein using the nuclear extraction kit (ab113474) and histone extraction kit (ab113476) respectively?

    Nuclear Protein extraction and Histone protein extraction from cell samples frozen at -80oC could still work using ab113474 and ab113476. However, the enzyme activity of nuclear extracts from frozen tissues may be much lower than that from fresh tissues.

    I am planning to extract histones from a kind of protozoan parasite, which is unicellular eukaryote. Is the ab113476 kit suitable for this isolation?

    Ab113476 can be used to extract histones from a protozoan parasite if you use the protocol for histone extraction from tissues.

    I am planning to extract histones from whole blood.
    Is the ab113476 from Abcam the suitable kit for this?

    yes ab113476 can be used for extracting histone proteins from frozen whole blood cells. For the best results, the amount of leukocyte cells in the whole blood should be estimated so that proper volume of whole blood can be used for extracting sufficien...

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    Base on the same principles the ab115102 may be suitable to use in fish too?



    Also I'm interesting in ab133099 and ab113476 it may funtion?

    The two kits ab115102 and ab113476 should be suitable for use in fish, while ab133099 is designed to study inhibitors and so fish is not suitable.

    1-10 of 27 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"