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ab113476 is suitable for a quick preparation of total histone extracts from mammalian cells and tissue samples. The minimal amount of starting materials can be as low as 105 cells or 1 mg of tissue. For the best results, the cell number should be greater than 106 cells or the tissue amount should be greater than 10 mg. A total of 100 standard extractions (use 107 cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 107 cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.
Shipped at +4°C. Store refrigerated at +4°C before preparation.
|10X Pre-Lysis Buffer||1 x 10ml|
|Balance Buffer||1 x 8ml|
|DTT Solution||1 x 20µl|
|Lysis Buffer||1 x 20ml|
Our Abpromise guarantee covers the use of ab113476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. Total histone protein yield = 0.4mg/107 or 100mg tissue (yield may vary depending on cell or tissue type).|
Histones were purified from mouse liver cells using ab113476. Each lane was loaded with 15μg of purified histone.
Image courtesy of Kevin Dalgaard, MPI for Immunobiology & Epigenetics, Freiburg, DE