Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457)

製品の概要

  • 製品名
    Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit
    KDM1 / LSD1 キット 製品一覧
  • アッセイタイプ
    Enzyme activity
  • 全工程の試験時間
    3h 00m
  • 種交差性
    交差種: Mouse, Human, Plants
    交差が予測される動物種: Mammal, Other
  • 製品の概要

    Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) allows the user to measure activity/inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.


    KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

  • 保存方法
    Please refer to protocols.
  • 内容 48 tests 96 tests
    10X Wash Buffer 1 x 15ml 1 x 30ml
    8-Well Assay Strips (with Frame) 6 units 12 units
    Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl
    Detection Antibody, 200 µg/mL 1 x 8µl 1 x 16µl
    Fluoro-Developer 1 x 6ml 1 x 12µl
    LSD1 Assay Buffer 1 x 2ml 1 x 4ml
    LSD1 Assay Standard, 20 µg/mL 1 x 25µl 1 x 50µl
    LSD1 Substrate 1 x 50µl 1 x 100µl
  • 研究分野
  • 関連性
    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2)H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor(ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
  • 別名
    • Amine oxidase (flavin containing) domain 2
    • AOF2
    • BHC110
    • BRAF35-HDAC complex protein BHC110
    • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
    • Flavin-containing amine oxidase domain-containing protein 2
    • KDM1
    • KIAA0601
    • LSD1
    • Lysine (K) specific demethylase 1
    • Lysine (K) specific demethylase 1A
    • Lysine-specific histone demethylase 1
    • Lysine-specific histone demethylase 1A
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • LSD1 activity measured from NIH-3T3 cell extracts.
  • Image B: B: RFU data after conversion from A.
  • LSD1 activity measurement with kit ab113457. Recombinant human LSD1 enzyme was added at different concentrations.
    Image A: Original RFU data. RFU is inversely proportional to LSD1 enzyme activity.

プロトコール

参考文献

ab113457 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting Abcam. For ab113459 and ab113460, both kits directly detects KDM1/LSD1-converted demethylated products, rather than by-products, in a 96 stripwell microplate format. The main difference between the 2 are that ab113459, has a co...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録