Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352)

製品の概要

  • 製品名Histone Acetyltransferase Activity Assay Kit (Colorimetric)
    Histone acetyltransferase キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Tissue Extracts, Cell Lysate
  • アッセイタイプEnzyme activity
  • 種交差性
    交差種: Mouse, Human, Zebrafish, all Mammals
  • 製品の概要

    Abcam's Histone Acetyltransferase Activity Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Histone acetyltransferases (HAT) are enzymes that acetylate conserved lysine amino acids on histone proteins. They have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation.
  • 細胞内局在Cytoplasmic and Nuclear. Note=Nuclear in S-phase cells and cytoplasmic.
  • 別名
    • HAT
    • HAT1
    • Histone acetyltransferase 1
    • Histone acetyltransferase type B catalytic subunit
    • KAT1
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab65352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Histone Acetyltransferase Activity Assay Kit (Colorimetric) 画像

  • Analysis of Histone Acetyltransferase Activity in HeLa Nuclear Extract using ab65352. HeLa nuclear extract in various amounts was incubated with HAT substrate. Activity was analyzed in a micro plate reader at 440 nm according to the kit instructions.

プロトコール

Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352) 使用論文

This product has been referenced in:
  • Goriki A  et al. A novel protein, CHRONO, functions as a core component of the mammalian circadian clock. PLoS Biol 12:e1001839 (2014). Read more (PubMed: 24736997) »
  • Kim JH  et al. Histone acetylation level and histone acetyltransferase/deacetylase activity in ejaculated sperm from normozoospermic men. Yonsei Med J 55:1333-40 (2014). Functional Studies ; Human . Read more (PubMed: 25048493) »

See all 10 Publications for this product

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Rat liver nuclear fraction

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Abreviews
Our samples were not within the linear range for the booklet's recommended protein concentration. A preliminary serial dilution curve should be prepared prior to running the full assay so as to ensure OD values are within the linear range.
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投稿 Apr 01 2015

You can use a background control sample, also use a nuclear extract.

I have had the chance to inquire to the lab about the use of a purified IP sample. Theywould suggest using the initial sample without any IP for the HAT assay. If the IP is just for concentrating the sample, they recommend use of spin columns, not IP. ...

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The lab has confirmed that this kit has not been validated for measuring Tubulin Acetyltransferase activity.  We have no information about whether it would be suitable for this purpose, but the kit was specifically designed for measuring Histone Acetyl...

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Thank you very much for your inquiry. 1.) I can confirm that the HAT activity can be expressed as the relative O.D. value per µg or nmol/min/µg sample. Please have a look at the following example calculation: A. Relative OD/ug: One obtains...

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DDT should be avoided, as this compound strongly interferes with the reactions in the kit. There are a few things I would like to suggest: - After acid extracting the histones in your current protocol, the sample could be dialysed overnight in 1...

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As long as the tissue samples were frozen appropriately, nuclear lysates generated from these tissues can be used for the HAT assay.

The feedback from the lab is that technically BME should be fine but we have not tested this specifically.

Thank you for your enquiry. I have obtained the following information to help answer your questions: 1) Each kit can test 50 samples. i.e. 100/2, minus the wells for background and the Hela nuclear extract used for a standard 2) If you use a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"