製品の概要

  • 製品名Anti-Histidine decarboxylase antibody
    Histidine decarboxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Histidine decarboxylase
  • 特異性Detects both the 74 kDa full length and the 54 kDa active form of Histidine decarboxylase.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Histidine decarboxylase. synthetic peptide around aa 22
    (Peptide available as ab55801)

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21873469
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with Histidine decarboxylase peptide (ab55801).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-Histidine decarboxylase antibody 画像



  • Predicted band size : 74 kDa
  • IHC image of ab37291 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37291, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histidine decarboxylase antibody (ab37291) 使用論文

This product has been referenced in:
  • Nizamutdinova IT  et al. Involvement of histamine in endothelium-dependent relaxation of mesenteric lymphatic vessels. Microcirculation 21:640-8 (2014). Read more (PubMed: 24750494) »
  • Francis H  et al. Inhibition of histidine decarboxylase ablates the autocrine tumorigenic effects of histamine in human cholangiocarcinoma. Gut 61:753-64 (2012). ICC/IF ; Human . Read more (PubMed: 21873469) »

See all 5 Publications for this product

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Thank you very much for your call today and for letting us know about the trouble with these antibodies.
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Thank you for calling Abcam earlier today.

I am sorry that you were having some problems using ab37291 in western blot. As we discussed on the telephone, I am sending you a new vial of this this to try out. Your new order number is ********. ...

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Thank you for your reply. I am happy to offer you a replacement product. I can arrange this through the distributor - please provide the distributor name. Also please indicate if you are okay to receive another vial of this antibody or a credit. ...

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Thank you for your reply. In order to send a replacement I will need a specific order number. Did you order the item directly from Abcam or through a distributor? I look forward to your reply so that I may assist you further.

Thank you for your reply. I can see that my colleagues are assisting you with ab3611 and ab15323. The protocol suggestions that I have given you are specific for ab37291 and I see that they have also offered protocol suggestions for these products. ...

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Thank you for contacting Abcam regarding ab37291. I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions to improve your resu...

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