製品の概要
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製品名Anti-HIPPI antibody
HIPPI 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HIPPI
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由来種Rabbit
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特異性This antibody has been shown to detect 0.1 ug/lane of recombinant human HIPPI. It also detects a band of the expected size when tested on a yeast lysate containing a HIPPI fusion protein.
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アプリケーション適用あり: WB, ICC/IF, ChIPmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HIPPI.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following tissue lysates: Human Heart Human Brain Mouse Brain This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma) A431 (Human epithelial carcinoma) MCF7 (Human breast adenocarcinoma) HEK293 (Human embryonic kidney) NIH 3T3 (Mouse embryonic fibroblast) MEF1 (Mouse embryonic fibroblast) This antibody gave a positive signal in the following nuclear lysates: HeLa Nuclear
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab5205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| ChIP | Use at an assay dependent concentration. PubMed: 21832040 |
ターゲット情報
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機能Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
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組織特異性Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
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配列類似性Belongs to the IFT57 family.
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ドメインThe pseudo DED region (pDED) meadiates the interaction with HIP1.
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細胞内局在Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.
- Information by UniProt
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参照データベース
- Entrez Gene: 55081 Human
- Entrez Gene: 73916 Mouse
- Omim: 606621 Human
- SwissProt: Q9NWB7 Human
- SwissProt: Q8BXG3 Mouse
- Unigene: 412196 Human
- Unigene: 241276 Mouse
- Unigene: 404371 Mouse
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別名
- CDNA FLJ10147 fis clone HEMBA1003369 antibody
- Dermal papilla derived protein 8 antibody
- Dermal papilla-derived protein 8 antibody
see all
画像
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Western blot - Anti-HIPPI antibody (ab5205)All lanes : Anti-HIPPI antibody (ab5205) at 1/1000 dilution
Lane 1 : Recombinant human HIPPI at 10 µg
Lane 2 : Recombinant human HIPPI at 1 µg
Lane 3 : Recombinant human HIPPI at 0.1 µg
Lane 4 : yeast extract + AD-HIPPI fusion protein
Lane 5 : yeast extract
Lane 6 : human lymphoblastoid extract at 10 µg
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 45 kDa (why is the actual band size different from the predicted?)Western blot using ab5205 at 1/1000 for 1hr in TBST.
Lane 1: recombinant human HIPPI (10 ug)
Lane 2: recombinant human HIPPI (1 ug)
Lane 3: recombinant human HIPPI (0.1 ug)
Lane 4: yeast extract + AD-HIPPI fusion protein
Lane 5: yeast extract
Lane 6: human lymphoblastoid extract (10ug)The indicated band in lane 4 is higher than the recombinant human HIPPI as it is a fusion protein with a Activation Domain.
The indicated band in lane 4 is higher than the recombinant human HIPPI as it is a fusion protein with a Activation Domain. -
Immunocytochemistry/ Immunofluorescence - Anti-HIPPI antibody (ab5205)ICC/IF image of ab5205 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5205, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Western blot - Anti-HIPPI antibody (ab5205)All lanes : Anti-HIPPI antibody (ab5205) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 :A431 whole cell lysate (ab7909)
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 :HEK293 whole cell lysate (ab7902)
Lane 7 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 43,49 kDa (why is the actual band size different from the predicted?)
This antibody recognizes a band at approximately 49 kDa in tissue lysates, however in whole cell lysates the protein appears to be running at 43 kDa. In previous studies this 43 kDa band has been blocked with the immunizing peptide (data not shown). -
Western blot - Anti-HIPPI antibody (ab5205)All lanes : Anti-HIPPI antibody (ab5205) at 1 µg/ml
Lane 1 :NIH 3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 43,49 kDa (why is the actual band size different from the predicted?)
This antibody recognizes a band at approximately 49 kDa in tissue lysates, however in whole cell lysates the protein appears to be running at 43 kDa.
参考文献
This product has been referenced in:
- Datta M & Bhattacharyya NP Regulation of RE1 Protein Silencing Transcription Factor (REST) Expression by HIP1 Protein Interactor (HIPPI). J Biol Chem 286:33759-69 (2011). ChIP, IP . Read more (PubMed: 21832040) »
- Banerjee M et al. Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1. Nucleic Acids Res 38:878-92 (2010). WB ; Human . Read more (PubMed: 19934260) »
レビューと Q&A
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