Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HIPPI.
Our Abpromise guarantee covers the use of ab5205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. | |
ICC/IF | Use a concentration of 5 µg/ml. | |
ChIP | Use at an assay dependent concentration. PubMed: 21832040 |
Western blot using ab5205 at 1/1000 for 1hr in TBST.
Lane 1: recombinant human HIPPI (10 ug)
Lane 2: recombinant human HIPPI (1 ug)
Lane 3: recombinant human HIPPI (0.1 ug)
Lane 4: yeast extract + AD-HIPPI fusion protein
Lane 5: yeast extract
Lane 6: human lymphoblastoid extract (10ug)
The indicated band in lane 4 is higher than the recombinant human HIPPI as it is a fusion protein with a Activation Domain.
The indicated band in lane 4 is higher than the recombinant human HIPPI as it is a fusion protein with a Activation Domain.
ICC/IF image of ab5205 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5205, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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