製品の概要
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製品名Anti-HIPPI antibody
HIPPI 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HIPPI
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特異性This antibody has been shown to detect 0.1 ug/lane of recombinant human HIPPI. It also detects a band of the expected size when tested on a yeast lysate containing a HIPPI fusion protein.
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アプリケーション適用あり: WB, ICC/IF, ChIPmore details
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種交差性交差種: Mouse, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HIPPI.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following tissue lysates: Human Heart Human Brain Mouse Brain This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma) A431 (Human epithelial carcinoma) MCF7 (Human breast adenocarcinoma) HEK293 (Human embryonic kidney) NIH 3T3 (Mouse embryonic fibroblast) MEF1 (Mouse embryonic fibroblast) This antibody gave a positive signal in the following nuclear lysates: HeLa Nuclear
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab5205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| ChIP | Use at an assay dependent concentration. PubMed: 21832040 |
ターゲット情報
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機能Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
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組織特異性Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
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配列類似性Belongs to the IFT57 family.
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ドメインThe pseudo DED region (pDED) meadiates the interaction with HIP1.
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細胞内局在Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.
- Information by UniProt
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参照データベース
- Entrez Gene: 55081 Human
- Entrez Gene: 73916 Mouse
- Omim: 606621 Human
- SwissProt: Q9NWB7 Human
- SwissProt: Q8BXG3 Mouse
- Unigene: 412196 Human
- Unigene: 241276 Mouse
- Unigene: 404371 Mouse
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別名
- CDNA FLJ10147 fis clone HEMBA1003369 antibody
- Dermal papilla derived protein 8 antibody
- Dermal papilla-derived protein 8 antibody
see all
Anti-HIPPI antibody 画像
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Western blot - Anti-HIPPI antibody (ab5205)All lanes : Anti-HIPPI antibody (ab5205) at 1/1000 dilution
Lane 1 : Recombinant human HIPPI at 10 µg
Lane 2 : Recombinant human HIPPI at 1 µg
Lane 3 : Recombinant human HIPPI at 0.1 µg
Lane 4 : yeast extract + AD-HIPPI fusion protein
Lane 5 : yeast extract
Lane 6 : human lymphoblastoid extract at 10 µg
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
The indicated band in lane 4 is higher than the recombinant human HIPPI as it is a fusion protein with a Activation Domain. -
Immunocytochemistry/ Immunofluorescence - HIPPI antibody (ab5205)ICC/IF image of ab5205 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5205, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Western blot - HIPPI antibody (ab5205)All lanes : Anti-HIPPI antibody (ab5205) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 :A431 whole cell lysate (ab7909)
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 :HEK293 whole cell lysate (ab7902)
Lane 7 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 43,49 kDa (why is the actual band size different from the predicted?) -
Western blot - HIPPI antibody (ab5205)All lanes : Anti-HIPPI antibody (ab5205) at 1 µg/ml
Lane 1 :NIH 3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 43,49 kDa (why is the actual band size different from the predicted?)
Anti-HIPPI antibody (ab5205) 使用論文
This product has been referenced in:
- Datta M & Bhattacharyya NP Regulation of RE1 Protein Silencing Transcription Factor (REST) Expression by HIP1 Protein Interactor (HIPPI). J Biol Chem 286:33759-69 (2011). ChIP, IP . Read more (PubMed: 21832040) »
- Banerjee M et al. Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1. Nucleic Acids Res 38:878-92 (2010). WB ; Human . Read more (PubMed: 19934260) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"