製品の概要

  • 製品名Anti-HIPPI antibody
    HIPPI 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HIPPI
  • 特異性This antibody has been shown to detect 0.1 ug/lane of recombinant human HIPPI. It also detects a band of the expected size when tested on a yeast lysate containing a HIPPI fusion protein.
  • アプリケーション適用あり: WB, ICC/IF, ChIPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HIPPI.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human Heart Human Brain Mouse Brain This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma) A431 (Human epithelial carcinoma) MCF7 (Human breast adenocarcinoma) HEK293 (Human embryonic kidney) NIH 3T3 (Mouse embryonic fibroblast) MEF1 (Mouse embryonic fibroblast) This antibody gave a positive signal in the following nuclear lysates: HeLa Nuclear

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.
ICC/IF Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 21832040

ターゲット情報

  • 機能Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
  • 組織特異性Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
  • 配列類似性Belongs to the IFT57 family.
  • ドメインThe pseudo DED region (pDED) meadiates the interaction with HIP1.
  • 細胞内局在Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.
  • Information by UniProt
  • 参照データベース
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Anti-HIPPI antibody 画像

  • All lanes : Anti-HIPPI antibody (ab5205) at 1/1000 dilution

    Lane 1 : Recombinant human HIPPI at 10 µg
    Lane 2 : Recombinant human HIPPI at 1 µg
    Lane 3 : Recombinant human HIPPI at 0.1 µg
    Lane 4 : yeast extract + AD-HIPPI fusion protein
    Lane 5 : yeast extract
    Lane 6 : human lymphoblastoid extract at 10 µg


    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    The indicated band in lane 4 is higher than the recombinant human HIPPI as it is a fusion protein with a Activation Domain.
  • ICC/IF image of ab5205 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5205, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • All lanes : Anti-HIPPI antibody (ab5205) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 4 : A431 whole cell lysate (ab7909)
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : HEK293 whole cell lysate (ab7902)
    Lane 7 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 43,49 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HIPPI antibody (ab5205) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 43,49 kDa (why is the actual band size different from the predicted?)

Anti-HIPPI antibody (ab5205) 使用論文

This product has been referenced in:
  • Datta M & Bhattacharyya NP Regulation of RE1 Protein Silencing Transcription Factor (REST) Expression by HIP1 Protein Interactor (HIPPI). J Biol Chem 286:33759-69 (2011). ChIP, IP . Read more (PubMed: 21832040) »
  • Banerjee M  et al. Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1. Nucleic Acids Res 38:878-92 (2010). WB ; Human . Read more (PubMed: 19934260) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (Gradient Gel)
Sample Human Cell lysate - whole cell (HCT116)
Specification HCT116
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 18 2014

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Tissue lysate - nuclear (HCT116)
Specification HCT116
Total protein in input 100 µg
Username

Abcam user community

Verified customer

投稿 Feb 18 2014

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (Hela Cells)
Specification Hela Cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Real-time PCR
Negative control No DNA control
Username

Abcam user community

Verified customer

投稿 Jul 24 2012

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hela Cells)
Total protein in input 5000 µg
Specification Hela Cells
Immuno-precipitation step Protein G
Username

Dr. jerry fl

Verified customer

投稿 Jul 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela Cells)
Loading amount 50 µg
Specification Hela Cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"