製品の概要

  • 製品名Anti-HIPK4 antibody
    HIPK4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to HIPK4
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Cynomolgus Monkey, Rhesus monkey
  • 免疫原

    Recombinant fragment: RGSSWEEGEH LGASAEPLAI LQRDEDGPNI DNMTMEAERP DPELFDPSSC PGEWLSEPDC TLESVRGPRA QGLPPRRSHQ HGPPRGATSF LQHVTGHH, corresponding to amino acids 519-616 of Human HIPK4 (AAH34501) with a proprietory tag.

  • ポジティブ・コントロール
    • HIPK4 transfected 293T cell lysate. Recombinant tagged human HIPK4 fragment.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HIPK 4 antibody
    • HIPK4 antibody
    • HIPK4_HUMAN antibody
    • Homeodomain interacting protein kinase 4 antibody
    • Homeodomain-interacting protein kinase 4 antibody
    see all

Anti-HIPK4 antibody 画像

  • Anti-HIPK4 antibody (ab77651) at 1 µg/ml + Recombinant tagged human HIPK4 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 69 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HIPK4 antibody (ab77651) at 1 µg/ml

    Lane 1 : HIPK4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 69 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-HIPK4 antibody (ab77651) 使用論文

ab77651 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab77651.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"