製品の概要

  • 製品名Anti-HIPK3 antibody
    HIPK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HIPK3
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region between residues 850 and 900 of human HIPK3 (NM_005734)

  • ポジティブ・コントロール
    • Mouse and rat testis lysates. Human testis tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
IHC-P Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors.
  • 組織特異性Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated, but autophosphorylation is not required for catalytic activity.
    May be sumoylated.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Androgen receptor interacting nuclear protein kinase antibody
    • Androgen receptor-interacting nuclear protein kinase antibody
    • ANPK antibody
    • DYRK6 antibody
    • Fas interacting serine/threonine protein kinase antibody
    • Fas-interacting serine/threonine-protein kinase antibody
    • FIST antibody
    • FIST3 antibody
    • Hipk3 antibody
    • HIPK3_HUMAN antibody
    • homeodomain interacting protein kinase 3 antibody
    • Homeodomain-interacting protein kinase 3 antibody
    • Homolog of protein kinase YAK1 antibody
    • PKY antibody
    • RP24 297H17.5 antibody
    • YAK1 antibody
    see all

Anti-HIPK3 antibody 画像

  • ab72538 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72538 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab72538 at 10µg/ml staining HIPK3 in human testis by Immunohistochemistry using paraffin-embedded tissue.
  • All lanes : Anti-HIPK3 antibody (ab72538) at 1 µg/ml

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse testis lysate with immunising peptide
    Lane 3 : Rat testis lysate


    Predicted band size : 134 kDa
    Observed band size : 134 kDa
    Additional bands at : 100 kDa,50 kDa. We are unsure as to the identity of these extra bands.

Anti-HIPK3 antibody (ab72538) 使用論文

This product has been referenced in:
  • Huh JH  et al. Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer. Br J Cancer 109:452-61 (2013). Read more (PubMed: 23807165) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"