Anti-FIST 抗体 (ab72538)
Key features and details
- Rabbit polyclonal to FIST
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FIST antibody -
製品の詳細
Rabbit polyclonal to FIST -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human FIST. NM_005734
Database link: Q9H422-1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72538の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
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IHC-P |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). |
IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. -
組織特異性
Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated, but autophosphorylation is not required for catalytic activity.
May be sumoylated. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10114 Human
- Entrez Gene: 15259 Mouse
- Entrez Gene: 83617 Rat
- Omim: 604424 Human
- SwissProt: Q9H422 Human
- SwissProt: Q9ERH7 Mouse
- SwissProt: O88850 Rat
- Unigene: 201918 Human
see all -
別名
- Androgen receptor interacting nuclear protein kinase antibody
- Androgen receptor-interacting nuclear protein kinase antibody
- ANPK antibody
see all
画像
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ab72538 at 10µg/ml staining FIST in human testis by Immunohistochemistry using paraffin-embedded tissue.
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All lanes : Anti-FIST antibody (ab72538) at 1 µg/ml
Lane 1 : Mouse testis lysate
Lane 2 : Mouse testis lysate with immunising peptide
Lane 3 : Rat testis lysate
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab72538 は 2 報の論文で使用されています。
- Zhang H et al. ROR1 promotes the proliferation of endometrial cancer cells. Int J Clin Exp Pathol 10:10603-10610 (2017). PubMed: 31966402
- Huh JH et al. Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer. Br J Cancer 109:452-61 (2013). PubMed: 23807165