製品の概要

  • 製品名Anti-HIPK2 antibody [EPR3819]
    HIPK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3819] to HIPK2
  • アプリケーション適用あり: WB, IP, ICC, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human HIPK2.

  • ポジティブ・コントロール
    • HT-1080 and HepG2 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-HIPK2 antibody (Alexa Fluor® 488) [EPR3819] (ab199208)
    Anti-HIPK2 antibody (Alexa Fluor® 647) [EPR3819] (ab199491)

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 100, 130 kDa (predicted molecular weight: 131 kDa).
IP 1/20.
ICC 1/100 - 1/250.
Flow Cyt 1/1000 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
    • 組織特異性Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
    • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylated on tyrosines (By similarity). Autophosphorylated.
      Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
      Ubiquitinated by FBXO3, leading to rapid proteasome-dependent degradation. This degradation, but not ubiquitination, is prevented in the presence of PML.
    • 細胞内局在Nucleus > PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
    • Information by UniProt
    • 参照データベース
    • 別名
      • hHIPk 2 antibody
      • hHIPk2 antibody
      • HIPK 2 antibody
      • Hipk2 antibody
      • HIPK2_HUMAN antibody
      • Homeodomain interacting protein kinase 2 antibody
      • Homeodomain-interacting protein kinase 2 antibody
      • Nbak1 antibody
      • Nuclear body-associated kinase 1 antibody
      • PRO0593 antibody
      • Sialophorin tail-associated nuclear serine/threonine-protein kinase antibody
      • Stank antibody
      see all

    Anti-HIPK2 antibody [EPR3819] 画像

    • Overlay histogram showing HEK293 cells stained with ab108543 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108543, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-HIPK2 antibody [EPR3819] (ab108543) at 1/2000 dilution

      Lane 1 : HT-1080 cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 131 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa (possible isoform).

    Anti-HIPK2 antibody [EPR3819] (ab108543) 使用論文

    This product has been referenced in:
    • Cozza G  et al. Synthesis and properties of a selective inhibitor of homeodomain-interacting protein kinase 2 (HIPK2). PLoS One 9:e89176 (2014). IP ; Human . Read more (PubMed: 24586573) »
    • Dacheux E  et al. BRCA1-Dependent Translational Regulation in Breast Cancer Cells. PLoS One 8:e67313 (2013). WB ; Human . Read more (PubMed: 23805307) »

    See all 2 Publications for this product

    Product Wall

    There are 3 isoforms of this protein with mwt 131kDa, 100kDa and 128kDa. The antibody is detecting the 100kDa isoform in hepG2 lysates.


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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"