製品の概要
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製品名Anti-HIPK2 antibody
HIPK2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HIPK2
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由来種Rabbit
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特異性ab28507 recognises HIPK2. It may recognize isoform 1, 2 and 3 of HIPK2 as the immunogen sequence is present in all three isoforms. Please refer to the literature for more information on the three isforms. It does not recognize HIPK1, HIPK3, or HIPK4.
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アプリケーション適用あり: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, ChIPmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Zebrafish
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免疫原
Synthetic peptide derived from within residues 37 - 86 of human HIPK2.
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ポジティブ･コントロール
- HepG2 Cell Lysate, human heart tissue
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーConstituents: PBS, 2% Sucrose
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab28507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| ELISA | 1/62500. | |
| WB | Use a concentration of 0.06 µg/ml. Predicted molecular weight: 110 kDa.Can be blocked with Human HIPK2 peptide (ab111675). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ChIP | Use at an assay dependent concentration. PubMed: 23565059 |
ターゲット情報
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機能Protein kinase acting as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. Phosphorylation of TP53 may be mediated by a TP53-HIPK2-AXIN1 complex. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB (By similarity). Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.
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組織特異性Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.
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配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾Phosphorylated on tyrosines (By similarity). Autophosphorylated.
Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.
Ubiquitinated by FBXO3, leading to rapid proteasome-dependent degradation. This degradation, but not ubiquitination, is prevented in the presence of PML. -
細胞内局在Nucleus > PML body. Cytoplasm. Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
- Information by UniProt
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参照データベース
- Entrez Gene: 28996 Human
- Entrez Gene: 15258 Mouse
- Entrez Gene: 362342 Rat
- Entrez Gene: 387294 Zebrafish
- Omim: 606868 Human
- SwissProt: Q9H2X6 Human
- SwissProt: Q9QZR5 Mouse
- Unigene: 397465 Human
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別名
- hHIPk 2 antibody
- hHIPk2 antibody
- HIPK 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPK2 antibody (ab28507)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling HIPK2 with ab28507 at 1/100. A Cy3-conjugated donkey anti-rabbit (1/200) was used as the secondary antibody. Positive staining seen the nuclei of pinealocytes and intersticial cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - HIPK2. Right - Merge. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIPK2 antibody (ab28507)ab28507, at 4.0-8.0ug/ml, staining human HIPK2 in (Human heart, cells with positive label are Myocardial cells) by Immunohistochemistry, Paraffin embedded tissue. -
Western blot - Anti-HIPK2 antibody (ab28507)This image is courtesy of an anonymous AbreviewAll lanes : Anti-HIPK2 antibody (ab28507) at 1/10000 dilution
Lane 1 : Control - HeLa Whole Cell extract
Lane 2 : HIPK2 plasmid overexpression
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : AlexaFluor 680 goat anti-rabbit at 1/10000 dilution
Predicted band size: 110 kDaSamples were incubated in primary antibody for 14 hours at 4°C.
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Western blot - Anti-HIPK2 antibody (ab28507)Anti-HIPK2 antibody (ab28507) at 0.06 µg/ml + HepG2 Cell Lysate
Secondary
Rabbit Anti HIPK2 at 1/50000 dilution
Predicted band size: 110 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-HIPK2 antibody (ab28507)ICC/IF image of ab28507 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28507, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
This product has been referenced in:
- Oh HJ et al. Inhibition of the processing of miR-25 by HIPK2-Phosphorylated-MeCP2 induces NOX4 in early diabetic nephropathy. Sci Rep 6:38789 (2016). Read more (PubMed: 27941951) »
- Singh SP et al. HIF-1a Plays a Critical Role in the Gestational Sidestream Smoke-Induced Bronchopulmonary Dysplasia in Mice. PLoS One 10:e0137757 (2015). IHC-P, WB ; Mouse . Read more (PubMed: 26361040) »
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