製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to HIP55
  • アプリケーション
    適用あり: ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog
  • 免疫原

    Synthetic peptide:

    AANLSRNGPALQE

    , corresponding to N terminal amino acids 2-14 of Human HIP55.

  • ポジティブ・コントロール
    • Jurkat and 3T3 lysates.
  • 特記事項
    GenBank Accession Number – NP_054782.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 22450813
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa).

ターゲット情報

  • 機能
    Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
  • 配列類似性
    Belongs to the ABP1 family.
    Contains 1 ADF-H domain.
    Contains 1 SH3 domain.
  • ドメイン
    The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
  • 翻訳後修飾
    Degraded by caspases during apoptosis.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABP1 antibody
    • Actin-binding protein 1 antibody
    • Cervical mucin associated protein antibody
    • Cervical mucin-associated protein antibody
    • Cervical SH3P7 antibody
    • CMAP antibody
    • Dbnl antibody
    • DBNL_HUMAN antibody
    • Drebrin F antibody
    • drebrin like antibody
    • Drebrin like protein antibody
    • Drebrin-F antibody
    • Drebrin-like protein antibody
    • HIP 55 antibody
    • HIP-55 antibody
    • HPK1 interacting protein of 55 kDa antibody
    • HPK1-interacting protein of 55 kDa antibody
    • SH3 domain containing protein 7 antibody
    • SH3 domain-containing protein 7 antibody
    • SH3P7 antibody
    • src homology 3 domain containing protein HIP 55 antibody
    see all

画像

  • Anti-HIP55 antibody (ab2836) at 1 µg/ml + 3T3 cell lysate at 35 µg

    Predicted band size : 49 kDa
  • ab2836 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

参考文献

This product has been referenced in:
  • Santos MS  et al. Protein interactions of the vesicular glutamate transporter VGLUT1. PLoS One 9:e109824 (2014). Read more (PubMed: 25334008) »
  • Hepper I  et al. The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions. J Immunol 188:4590-601 (2012). ICC/IF ; Human . Read more (PubMed: 22450813) »

See all 3 Publications for this product

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