製品の概要

  • 製品名Anti-HIP2 antibody [EP1145Y]
    HIP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1145Y] to HIP2
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues of human HIP2.

  • ポジティブ・コントロール
    • Daudi cell lysate or human liver tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100000. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
IP 1/50.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
    • 組織特異性Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Belongs to the ubiquitin-conjugating enzyme family.
      Contains 1 UBA domain.
    • 翻訳後修飾Sumoylation at Lys-14 impairs catalytic activity.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E2 25K antibody
      • E2(25K) antibody
      • HIP-2 antibody
      • Huntingtin interacting protein 2 antibody
      • Huntingtin-interacting protein 2 antibody
      • HYPG antibody
      • LIG antibody
      • UBC1 antibody
      • UBE2K antibody
      • UBE2K_HUMAN antibody
      • Ubiquitin carrier protein antibody
      • Ubiquitin conjugating enzyme E2 25 kDa antibody
      • ubiquitin conjugating enzyme E2K antibody
      • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
      • Ubiquitin protein ligase antibody
      • Ubiquitin-conjugating enzyme E2 K antibody
      • Ubiquitin-conjugating enzyme E2(25K) antibody
      • Ubiquitin-conjugating enzyme E2-25 kDa antibody
      • Ubiquitin-conjugating enzyme E2-25K antibody
      • Ubiquitin-protein ligase antibody
      see all

    Anti-HIP2 antibody [EP1145Y] 画像

    • Anti-HIP2 antibody [EP1145Y] (ab52930) at 1/100000 dilution + Daudi cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 22 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    • Ab52930 (1:250) staining human HIP2 in human liver tissue by immunohistochemistry using paraffin embedded tissue.

    Anti-HIP2 antibody [EP1145Y] (ab52930) 使用論文

    This product has been referenced in:
    • Benedikt A  et al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia 25:135-44 (2011). WB ; Human . Read more (PubMed: 21030982) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Brain)
    Antigen retrieval step None
    Permeabilization Yes - 10% Tween-20
    Specification Brain
    Blocking step Heat-Inactivated Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

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    Verified customer

    投稿 Jan 22 2016

    The antibody ab52930 is raised against a peptide located within the range of amino acids 140 - 170 of human HIP2/E2-25K, http://www.uniprot.org/uniprot/P61086. There are no known issues of detection of SUMOylated samples. I am not sure what is happenin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"