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RabMAb

Anti-HIP2 抗体 [EP1144Y] (ab68162)

製品の概要

  • 製品名
    Anti-HIP2 antibody [EP1144Y]
    HIP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1144Y] to HIP2
  • アプリケーション
    適用あり: WB, IP, ICCmore details
    適用なし: Flow Cyt or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N-terminus of human HIP2.

  • ポジティブ・コントロール
    • Daudi, Jurkat, HepG2 and human brain cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
IP 1/20.
ICC 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IHC-P.
  • ターゲット情報

    • 機能
      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
    • 組織特異性
      Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the ubiquitin-conjugating enzyme family.
      Contains 1 UBA domain.
    • 翻訳後修飾
      Sumoylation at Lys-14 impairs catalytic activity.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E2 25K antibody
      • E2(25K) antibody
      • HIP-2 antibody
      • Huntingtin interacting protein 2 antibody
      • Huntingtin-interacting protein 2 antibody
      • HYPG antibody
      • LIG antibody
      • UBC1 antibody
      • UBE2K antibody
      • UBE2K_HUMAN antibody
      • Ubiquitin carrier protein antibody
      • Ubiquitin conjugating enzyme E2 25 kDa antibody
      • ubiquitin conjugating enzyme E2K antibody
      • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
      • Ubiquitin protein ligase antibody
      • Ubiquitin-conjugating enzyme E2 K antibody
      • Ubiquitin-conjugating enzyme E2(25K) antibody
      • Ubiquitin-conjugating enzyme E2-25 kDa antibody
      • Ubiquitin-conjugating enzyme E2-25K antibody
      • Ubiquitin-protein ligase antibody
      see all

    画像

    • All lanes : Anti-HIP2 antibody [EP1144Y] (ab68162) at 1/500 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : human brain cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 22 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    参考文献

    ab68162 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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