Anti-HIP2 抗体 [757C3a] (ab50837)

製品の概要

• 製品名
  Anti-HIP2 antibody [757C3a]
  HIP2 一次抗体 製品一覧
• 製品の詳細
  Mouse monoclonal [757C3a] to HIP2
• 特異性
  This antibody reacts with HIP2.
• アプリケーション
  適用あり: WB, ELISAmore details
• 種交差性
  交差種: Human
  
• 免疫原

  HIP2 recombinant fragment (Human), containing 50-200 AAs from near the C-terminal.

製品の特性

• 製品の状態
  Liquid
• 保存方法
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
• バッファー
  Preservative: 0.05% Sodium Azide
  Constituents: 1% BSA, PBS
• Concentration information loading...
• 精製度
  Protein G purified
• 特記事項（精製）
  Protein G purified from cultured supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through 0.22µm membrane.
• ポリ/モノ
  モノクローナル
• クローン名
  757C3a
• アイソタイプ
  IgG1
• 研究分野
  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

  • Epigenetics and Nuclear Signaling
  • Ubiquitin & Ubiquitin Like Modifiers
  • E2 Ubiquitin Conjugating Enzymes

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2 Enzymes

関連製品

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

アプリケーション

ターゲット情報

• 機能
  Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
• 組織特異性
  Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
• パスウェイ
  Protein modification; protein ubiquitination.
• 配列類似性
  Belongs to the ubiquitin-conjugating enzyme family.
  Contains 1 UBA domain.
• 翻訳後修飾
  Sumoylation at Lys-14 impairs catalytic activity.
• 細胞内局在
  Cytoplasm.

• Target information above from: UniProt accession P61086 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 3093 Human
  • Omim: 602846 Human
  • SwissProt: P61086 Human
  • Unigene: 50308 Human
  • Unigene: 725263 Human
• 別名
  • E2 25K antibody
  • E2(25K) antibody
  • HIP-2 antibody

  • Huntingtin interacting protein 2 antibody
  • Huntingtin-interacting protein 2 antibody
  • HYPG antibody
  • LIG antibody
  • UBC1 antibody
  • UBE2K antibody
  • UBE2K_HUMAN antibody
  • Ubiquitin carrier protein antibody
  • Ubiquitin conjugating enzyme E2 25 kDa antibody
  • ubiquitin conjugating enzyme E2K antibody
  • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
  • Ubiquitin protein ligase antibody
  • Ubiquitin-conjugating enzyme E2 K antibody
  • Ubiquitin-conjugating enzyme E2(25K) antibody
  • Ubiquitin-conjugating enzyme E2-25 kDa antibody
  • Ubiquitin-conjugating enzyme E2-25K antibody
  • Ubiquitin-protein ligase antibody

  see all

プロトコール

• Western blot protocols