HIP1R overexpression 293T lysate (whole cell) (ab94268)

製品の概要

  • 製品名
    HIP1R overexpression 293T lysate (whole cell)
  • 製品の詳細
    HIP1R overexpression lysate
  • 特記事項
    ab94268 is a 293T cell transfected lysate in which Human HIP1R has been transiently over-expressed using a pCMV-HIP1R plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • バッファー
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 精製度
    Whole Cell Lysate
  • 研究分野
  • 背景
    Function: Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. Tissue specificity: Brain, heart, kidney, pancreas, and liver, but not in lung or placenta. Similarity: Belongs to the SLA2 family. Contains 1 ENTH (epsin N-terminal homology) domain. Contains 1 I/LWEQ domain. Domain: Binds F-actin via the talin-like I/LWEQ domain.

アプリケーション

Our Abpromise guarantee covers the use of ab94268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

画像

  • ab94268 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-HIP1R antibody (ab88468) at 1/500 dilution

    Lane 1 : HIP1R overexpression 293T lysate (whole cell) (ab94268)
    Lane 2 : non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

参考文献

ab94268 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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