製品の概要
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製品名Anti-HIP1R antibody
HIP1R 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HIP1R
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human, Xenopus laevis
交差が予測される動物種: Mouse, Rat, Rabbit
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HIP1R.
(Peptide available as ab86622.)
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ポジティブ･コントロール
- This antibody gave a positive signal in Human Brain Tissue Lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab77297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 119 kDa (predicted molecular weight: 119 kDa). |
ターゲット情報
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機能Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
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組織特異性Brain, heart, kidney, pancreas, and liver, but not in lung or placenta.
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配列類似性Belongs to the SLA2 family.
Contains 1 ENTH (epsin N-terminal homology) domain.
Contains 1 I/LWEQ domain. -
ドメインBinds F-actin via the talin-like I/LWEQ domain.
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細胞内局在Cytoplasm > perinuclear region. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region.
- Information by UniProt
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参照データベース
- Entrez Gene: 9026 Human
- Entrez Gene: 29816 Mouse
- Entrez Gene: 81917 Rat
- Omim: 605613 Human
- SwissProt: O75146 Human
- SwissProt: Q9JKY5 Mouse
- Unigene: 524815 Human
- Unigene: 714965 Human
see all -
別名
- FLJ14000 antibody
- FLJ27022 antibody
- HIP-12 antibody
see all
画像
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Western blot - Anti-HIP1R antibody (ab77297)Anti-HIP1R antibody (ab77297) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 119 kDa
Exposure time: 5 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody (ab77297)ICC/IF image of ab77297 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77297, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody (ab77297)Xenopus laevis cytoplasmic egg extract visualized live with primary and secondary antibody addition [red is anti-Clathrin X22 (ab2731) with goat anti-mouse Alexa Fluor 568 secondary, green is anti-HIP1R (ab77297) with goat anti-rabbit Alexa Fluor 488 secondary]. HIP1R staining is specific for perinuclear membrane compartments.
参考文献
ab77297 has not yet been referenced specifically in any publications.
レビューと Q&A
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