製品の概要

  • 製品名Anti-HINT1 antibody [EPR5108]
    HINT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5108] to HINT1
  • アプリケーション適用あり: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human HINT1.

  • ポジティブ・コントロール
    • 293T, MCF7, HepG2 and Jurkat cell lysates; Human breast carcinoma and Human colonic adenocarcinoma tissues.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the HINT family.
    Contains 1 HIT domain.
  • ドメインThe histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
  • 細胞内局在Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenosine 5' monophosphoramidase antibody
    • Adenosine 5''-monophosphoramidase antibody
    • HINT 1 antibody
    • HINT1 antibody
    • HINT1_HUMAN antibody
    • Histidine triad nucleotide binding protein 1 antibody
    • Histidine triad nucleotide-binding protein 1 antibody
    • PKCI 1 antibody
    • PKCI-1 antibody
    • PKCI1 antibody
    • PRKCNH1 antibody
    • Protein kinase C inhibitor 1 antibody
    • Protein kinase C interacting protein 1 antibody
    • Protein kinase C-interacting protein 1 antibody
    see all

Anti-HINT1 antibody [EPR5108] 画像

  • All lanes : Anti-HINT1 antibody [EPR5108] (ab124912) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 14 kDa
  • ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded breast carcinoma tissue by immunohistochemistry.
  • ab124912, at 1/250 dilution, staining HINT1 in paraffin embedded colonic adenocarcinoma tissue by immunohistochemistry.

Anti-HINT1 antibody [EPR5108] (ab124912) 使用論文

ab124912 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab124912.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"