製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 23451260

ターゲット情報

  • 機能Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.
  • 組織特異性Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 翻訳後修飾In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation.
  • 細胞内局在Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic-helix-loop-helix-PAS protein MOP7 antibody
    • bHLHe17 antibody
    • Class E basic helix-loop-helix protein 17 antibody
    • HIF 3A antibody
    • HIF 3A4 antibody
    • HIF-3-alpha antibody
    • HIF3 alpha antibody
    • HIF3-alpha antibody
    • HIF3-alpha-1 antibody
    • HIF3A antibody
    • HIF3A_HUMAN antibody
    • Hypoxia Inducible Factor 3 alpha antibody
    • Hypoxia inducible factor 3 alpha subunit antibody
    • Hypoxia inducible factor three alpha antibody
    • Hypoxia-inducible factor 3-alpha antibody
    • Inhibitory PAS domain protein antibody
    • IPAS antibody
    • Member of PAS protein 7 antibody
    • MOP7 antibody
    • PAS domain-containing protein 7 antibody
    • PASD7 antibody
    see all

Anti-HIF3 alpha antibody 画像

  • Anti-HIF3 alpha antibody (ab2165) at 1/1600 dilution + 3T3 lysate treated with Cobalt (II) chloride at 10 µg/ml

    Secondary
    Goat-anti-Rabbit at 1/10000 dilution

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 24 kDa,55 kDa. We are unsure as to the identity of these extra bands.

Anti-HIF3 alpha antibody (ab2165) 使用論文

This product has been referenced in:
  • Huang Y  et al. Hypoxia inducible factor 3a plays a critical role in alveolarization and distal epithelial cell differentiation during mouse lung development. PLoS One 8:e57695 (2013). IHC-P ; Mouse . Read more (PubMed: 23451260) »
  • Huang C & Hales BF Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs. PLoS One 7:e51937 (2012). WB ; Mouse . Read more (PubMed: 23251655) »

See all 2 Publications for this product

Product Wall

The antibody recognises Hif-3 alpha. The sequence of the immunogen is EIKPPKRSPRLE conjugated to KLH, corresponding to amino acids 581 - 592 of Mouse HIF-3 alpha.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"