製品の概要

  • 製品名Anti-HIF1 beta antibody [2B10]
    HIF1 beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2B10] to HIF1 beta
  • 特異性Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).
  • アプリケーション適用あり: Flow Cyt, ICC/IF, ICC, IHC-P, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis, Fish, Non Human Primates, Zebrafish, African Green Monkey
    交差が予測される動物種: Rabbit, Cow
  • 免疫原

    Synthetic peptide corresponding to Human HIF1 beta aa 771-789.
    Sequence:

    NSYNNEEFPDLTMFPPFSE

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
EMSA Use at an assay dependent concentration.
ICC/IF 1/1000.
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB 1/4000. Detects a band of approximately 87 kDa (predicted molecular weight: 86 kDa).

ターゲット情報

  • 機能Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • arnT antibody
    • ARNT protein antibody
    • ARNT_HUMAN antibody
    • Aryl hydrocarbon receptor nuclear translocator antibody
    • bHLHe2 antibody
    • Class E basic helix-loop-helix protein 2 antibody
    • Dioxin receptor antibody
    • Dioxin receptor nuclear translocator antibody
    • Drnt antibody
    • HIF 1 beta antibody
    • HIF 1beta antibody
    • HIF-1-beta antibody
    • HIF1-beta antibody
    • HIF1B antibody
    • HIF1beta antibody
    • Hypoxia Inducible Factor 1 antibody
    • Hypoxia inducible factor 1 beta antibody
    • Hypoxia-inducible factor 1-beta antibody
    • nuclear translocator antibody
    • Tango antibody
    see all

Anti-HIF1 beta antibody [2B10] 画像

  • Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in A2058 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in HeLa cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in U251 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Anti-HIF1 beta antibody [2B10] (ab2771) at 1/2000 dilution + Human U2OS cells - whole cell lysate at 20 µg

    Secondary
    HRP conjugated goat anti-mouse antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 86 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible non-specific binding).

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC image of ab2771 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing PC12 cells stained with ab2771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HIF1 beta antibody [2B10] (ab2771) 使用論文

This product has been referenced in:
  • Zhao Y  et al. Chronic cerebral hypoperfusion causes decrease of O-GlcNAcylation, hyperphosphorylation of tau and behavioral deficits in mice. Front Aging Neurosci 6:10 (2014). WB ; Mouse . Read more (PubMed: 24575038) »
  • Lalevée S  et al. miR455 is linked to hypoxia signaling and is deregulated in preeclampsia. Cell Death Dis 5:e1408 (2014). Read more (PubMed: 25188518) »

See all 7 Publications for this product

Product Wall

While we do have publications using this antibody in mouse samples we are not presently aware of specific publications using mouse brain homogenates. HIF1 beta forms heterodimers with HIF1alpha and has ubiquitous nuclear expression. The HIF1 complex...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HOS cells)
Loading amount 50 µg
Specification HOS cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Mar 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293T)
Total protein in input 500 µg
Specification 293T
Immuno-precipitation step Protein G
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投稿 Oct 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount 30 µg
Specification Cos-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Sep 26 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount 30 µg
Specification 3T3 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Sep 26 2007

Thank you for your patience. I am sorry to hear that you are experiencing difficulties with this product ab2771 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (forebrain)
Loading amount 15 µg
Specification forebrain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Jun 22 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 20 µg
Specification PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Jun 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS)
Loading amount 20 µg
Specification U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Jun 15 2007

Thank you for your enquiry. Followingf corresponce with the source of this antibody I have determined that the cell lines used for IP were mouse Hep1c1c7 and human HeLa cells. ab2771 works well by IP in both mouse and human lysates at 1-2 ug/IP. ...

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