Anti-HIF Prolyl Hydroxylases 抗体 [EPR3664(2)] (ab109734)


  • 製品名Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)]
    HIF Prolyl Hydroxylases 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3664(2)] to HIF Prolyl Hydroxylases
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human HIF Prolyl Hydroxylases.

  • ポジティブ・コントロール
    • 293T, U87-MG, HeLa, HepG2, and SH-SY5Y cell lysates, Human brain tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab109734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
    • 組織特異性Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.
    • 配列類似性Contains 2 EF-hand domains.
      Contains 1 Fe2OG dioxygenase domain.
    • 翻訳後修飾Glycosylated.
    • 細胞内局在Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EGLN4 antibody
      • FLJ20262 antibody
      • HIF prolyl hydroxylase PH4 antibody
      • HIF-PH4 antibody
      • HIF-prolyl hydroxylase 4 antibody
      • HIFPH4 antibody
      • HPH-4 antibody
      • Hypoxia inducible factor prolyl 4 hydroxylase antibody
      • Hypoxia inducible factor prolyl hydroxylase antibody
      • Hypoxia-inducible factor prolyl hydroxylase 4 antibody
      • P4H TM antibody
      • P4H with transmembrane domain antibody
      • P4H-TM antibody
      • P4htm antibody
      • P4HTM_HUMAN antibody
      • PH 4 antibody
      • PH4 antibody
      • PHD4 antibody
      • Proline 4 hydroxylase antibody
      • Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) antibody
      • Prolyl hydroxlase domain containing 4 antibody
      • Transmembrane prolyl 4-hydroxylase antibody
      see all

    Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] 画像

    • All lanes : Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 57 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109734 at a dilution of 1/100.

    Anti-HIF Prolyl Hydroxylases antibody [EPR3664(2)] (ab109734) 使用論文

    ab109734 has not yet been referenced specifically in any publications.

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