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Fusion protein (proprietary-tag) corresponding to Human HIF-2-alpha aa 535-631.
Our Abpromise guarantee covers the use of ab8365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/1000 - 1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Detects a band of approximately 118 kDa (predicted molecular weight: 100 kDa).|
Immunohistochemical analysis of Human breast cancer tissue, staining HIF-2-alpha with ab8365.
Antigen retrieval was performed using EDTA buffer in a pressure cooker, before being blocked with normal serum. Samples were incubated with primary antibody (1/2000) overnight at 4°C. Staining was detected using DAB.
ab8365 at 1/300 dilution, staining HIF-2-alpha in cardiomyocytes of Human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
HIF-2-alpha detected in hypoxic Human lysate using ab8365. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.