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Synthetic peptide corresponding to Human HIF-1 alpha.
Our Abpromise guarantee covers the use of ab72775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Predicted molecular weight: 93 kDa.|
ICC/IF image of ab72775 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72775, 1/50 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Image from Wiley M et al., J Biol Chem. 2010 Aug 27;285(35):26852-60. Epub 2010 Jun 25. Fig 2.; doi: 10.1074/jbc.M110.147041; August 27, 2010 The Journal of Biological Chemistry, 285, 26852-26860.Representative western blot detecting HIF1 alpha (Hydroxy P402) with ab72775; a different antibody was used to detect total HIF1 alpha and an antibody against actin was used as a loading control.