製品の概要

  • 製品名HIF-1-alpha antibody panel
  • 種交差性
    交差種: Mouse, Rat, Human
  • 製品の概要

    This panel contains 50µl of the monoclonals and 25µl of the polyclonals of the following antibodies:

     

    Mab1 - ab463 HIF-1-alpha mouse monoclonal antibody; Clone H1alpha67; Isotype = IgG2b; Purity = Protein G; Concentration = 2.0mg/ml.

     

    Mab2 - ab8366 HIF-1-alpha mouse monoclonal antibody; Clone ESEE122; Isotype = IgG1; Purity = Protein G; Concentration = 1.0mg/ml.

     

    Pab1 - HIF-1-alpha rabbit polyclonal antibody (amino acids 530-825); affinity purified;Concentration = 1.0mg/ml.

     

    Pab2 - HIF-1-alpha rabbit polyclonal antibody (C-term);immunogen affinity purified;Concentration = 0.2mg/ml.

     

    These antibodies are specific for HIF-1-alpha.

  • アプリケーション適用あり: IHC-P, IHC-Fr, WBmore details

製品の特性

  • 機能Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
  • 組織特異性Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • ドメインContains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).
  • 翻訳後修飾In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
    In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
    S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
    Requires phosphorylation for DNA-binding.
    Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
    Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
  • Information by UniProt
  • 別名
    • ARNT interacting protein
    • ARNT-interacting protein
    • Basic helix loop helix PAS protein MOP1
    • Basic-helix-loop-helix-PAS protein MOP1
    • bHLHe78
    • Class E basic helix-loop-helix protein 78
    • HIF 1A
    • HIF 1alpha
    • HIF-1-alpha
    • HIF1
    • HIF1 A
    • HIF1 Alpha
    • HIF1-alpha
    • HIF1A
    • HIF1A_HUMAN
    • Hypoxia inducible factor 1 alpha
    • Hypoxia inducible factor 1 alpha isoform I.3
    • Hypoxia inducible factor 1 alpha subunit
    • Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor
    • Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor)
    • Hypoxia inducible factor1alpha
    • Hypoxia-inducible factor 1-alpha
    • Member of PAS protein 1
    • Member of PAS superfamily 1
    • Member of the PAS Superfamily 1
    • MOP 1
    • MOP1
    • PAS domain-containing protein 8
    • PASD 8
    • PASD8
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab10625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background.
IHC-Fr 1/100 - 1/500. A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background.(only ab8366 has been used on frozen sections). Antigen retrieval is strongly recommended.
WB Use at an assay dependent dilution. Predicted molecular weight: 93 kDa. These antibodies work for Western blot analysis but are not specifically recommended for this application.

HIF-1-alpha antibody panel (ab10625) 使用論文

This product has been referenced in:
  • Miyazawa K  et al. Immunohistochemical expression of four different stem cell markers in prostate cancer: High expression of NANOG in conjunction with hypoxia-inducible factor-1a expression is involved in prostate epithelial malignancy. Oncol Lett 8:985-992 (2014). IHC-P ; Human . Read more (PubMed: 25120646) »
  • Chávez JC  et al. Expression of hypoxia-inducible factor-1alpha in the brain of rats during chronic hypoxia. J Appl Physiol 89:1937-42 (2000). Read more (PubMed: 11053346) »

See all 3 Publications for this product

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Thank you for your message and for your patience.

I have copied below western blot testing protocols for 3 of the antibodies included in this panel of 4 antibodies. Please note that these protocols are a recommendation only, and may require ...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from the ab463 Anti-HIF1 alpha antibodyfrom this panel.

The details you have kindly provide...

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Thank you for contacting Abcam. I have had a chance to look through the data for at HIF1 alpha antibody panel (ab10625) that you had spoke of during our conversation. Of the antibodies included in this panel only ab8366 (Anti-HIF1 alpha antibody [...

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Thank you for your enquiry. We have found that certain HIF antibodies work in one person's hands but not in another, and vice-versa. The ab10625 contains 2 different HIF abs that work in IHC and the ab6679 contains 4 different HIF abs that work in W...

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I'm sorry you are having problems with this kit and ab1, and take your complaint very seriously. I would like to know if those products were shipped to you together, and whether they were bought from a distributor or through Abcam directly. This inf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"