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Synthetic peptide corresponding to Human HIF-1 alpha aa 239-530.
Our Abpromise guarantee covers the use of ab6489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 93 kDa).|
ab6489 staining chicken embryo myocardium tissue sections by IHC-Fr. Sections were fixed in Zn fixative and permeablized in 0.2% Triton X-100 in PBS prior to blocking in 5% serum for 1 hour at 37°C. Ab6489 was diluted 1/200 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 555 conjugated donkey anti-mouse antibody was used as the secondary.