製品の概要

  • 製品名Anti-HIF-1-alpha antibody
    HIF-1-alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HIF-1-alpha
  • アプリケーション適用あり: IHC-P, ICCmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Carp, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human HIF-1-alpha aa 775-826 (C terminal).

  • ポジティブ・コントロール
    • Mouse squamous cell carcinoma tissue; HeLa cells.
  • 特記事項

    Concentration is optimized for IHC and not determined

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab114977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
ICC 1/100 - 1/500.

ターゲット情報

  • 機能Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP.
  • 組織特異性Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • ドメインContains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID).
  • 翻訳後修飾In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
    In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
    S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
    Requires phosphorylation for DNA-binding.
    Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
    Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARNT interacting protein antibody
    • ARNT-interacting protein antibody
    • Basic helix loop helix PAS protein MOP1 antibody
    • Basic-helix-loop-helix-PAS protein MOP1 antibody
    • bHLHe78 antibody
    • Class E basic helix-loop-helix protein 78 antibody
    • HIF 1A antibody
    • HIF 1alpha antibody
    • HIF-1-alpha antibody
    • HIF1 A antibody
    • HIF1 Alpha antibody
    • HIF1 antibody
    • HIF1-alpha antibody
    • HIF1A antibody
    • HIF1A_HUMAN antibody
    • Hypoxia inducible factor 1 alpha antibody
    • Hypoxia inducible factor 1 alpha isoform I.3 antibody
    • Hypoxia inducible factor 1 alpha subunit antibody
    • Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody
    • Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody
    • Hypoxia inducible factor1alpha antibody
    • Hypoxia-inducible factor 1-alpha antibody
    • Member of PAS protein 1 antibody
    • Member of PAS superfamily 1 antibody
    • Member of the PAS Superfamily 1 antibody
    • MOP 1 antibody
    • MOP1 antibody
    • PAS domain-containing protein 8 antibody
    • PASD 8 antibody
    • PASD8 antibody
    see all

Anti-HIF-1-alpha antibody 画像

  • ab114977 at 1:250 dilution staining HIF-1-alpha in Formaldehyde-fixed asynchronous hypoxic HeLa cells by Immunocytochemistry.
    Detection: DAB staining.

  • ab114977 at 1:250 dilution staining HIF-1-alpha in Formalin-fixed, Paraffin-embedded Mouse squamous cell carcinoma tissue by Immunohistochemistry.
    Detection: DAB staining.

Anti-HIF-1-alpha antibody (ab114977) 使用論文

This product has been referenced in:
  • Chen F  et al. Proteome Differences in Placenta and Endometrium between Normal and Intrauterine Growth Restricted Pig Fetuses. PLoS One 10:e0142396 (2015). WB ; Pig . Read more (PubMed: 26554841) »
  • Ronkainen VP  et al. Hypoxia-inducible factor 1-induced G protein-coupled receptor 35 expression is an early marker of progressive cardiac remodelling. Cardiovasc Res N/A:N/A (2013). Read more (PubMed: 24095869) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Murine Prostate Tumor Cells)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Treatment Cobalt Chloride 100uM - 300uM
Specification Murine Prostate Tumor Cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Yu Zheng

Verified customer

投稿 Aug 19 2015

Thank you for contacting us and your interest in our products.
The anti-HIF1 alpha antibody ab114977 does not have a concentration provided as it is specifically prepared to provide optimum IHC staining within the recommended range of dilutions. T...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"