製品の概要

  • 製品名Anti-HIC5 antibody
    HIC5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HIC5
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (PRSGAPKERP AEPLTPPPSY GHQPQTGSGE SSGASGDKDH LYSTVCKPRS) of human HIC5 (NP_057011).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.
  • 組織特異性Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).
  • 配列類似性Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • ドメインThe LIM zinc-binding domains mediate glucocorticoid receptor coactivation and interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 and SMAD3. The LIM zinc-binding 2 and LIM zinc-binding 3 domains mediate targeting to focal adhesions and actin stress fibers. The LIM zinc-binding 3 and LIM zinc-binding 4 domains mediate interaction with TRAF4 and MAPK15. The LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization and targeting to the nuclear matrix. The LIM zinc-binding 3 domain mediates interaction with PTPN12.
    The LD (leucine and aspartate-rich) motif 3 mediates interaction with GIT1 and functions as a nuclear export signal.
  • 翻訳後修飾Phosphorylated by gonadotropin-releasing hormone-activated SRC.
  • 細胞内局在Cell junction > focal adhesion. Nucleus matrix. Cytoplasm > cytoskeleton. Associated with the actin cytoskeleton; colocalizes with stress fibers.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Androgen receptor coactivator 55 kDa protein antibody
    • Androgen receptor coactivator ARA55 antibody
    • Androgen receptor-associated protein of 55 kDa antibody
    • ARA55 antibody
    • Hic-5 antibody
    • Hydrogen peroxide-inducible clone 5 protein antibody
    • Tgfb1i1 antibody
    • TGFI1_HUMAN antibody
    • Transforming growth factor beta 1 induced transcript 1 antibody
    • Transforming growth factor beta-1-induced transcript 1 protein antibody
    • TSC 5 antibody
    see all

Anti-HIC5 antibody 画像

  • Anti-HIC5 antibody (ab83273) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling HIC5 with ab83273 at 5.0µg/ml.

Anti-HIC5 antibody (ab83273) 使用論文

ab83273 has not yet been referenced specifically in any publications.

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