製品の概要

  • 製品名Anti-HIC1 antibody
    HIC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HIC1
  • 特異性This antibody is specific for HIC1.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A region within synthetic peptide: ALCASERRCS PLCGLDLSKK SPPGSAAPER PLAERELPPR PDSPPSAGPA, corresponding to amino acids 228-277 of Human HIC1

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

ターゲット情報

  • 機能Transcriptional repressor. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall.
  • 組織特異性Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
  • 配列類似性Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily.
    Contains 1 BTB (POZ) domain.
    Contains 5 C2H2-type zinc fingers.
  • 翻訳後修飾Acetylated on several residues, including Lys-333. Lys-333 is deacetylated by SIRT1.
    Sumoylated on Lys-333 by a PIAS family member, which positively regulates transcriptional repression ativity and is enhanced by HDAC4.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Hic 1 antibody
    • Hic-1 antibody
    • Hic1 antibody
    • HIC1_HUMAN antibody
    • Hypermethylated in cancer 1 antibody
    • Hypermethylated in cancer 1 protein antibody
    • ZBTB29 antibody
    • Zinc finger and BTB domain-containing protein 29 antibody
    • ZNF901 antibody
    see all

Anti-HIC1 antibody 画像

  • Lane 1 : MARKER
    Lane 2 : Anti-HIC1 antibody (ab49326) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 1 :
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)

Anti-HIC1 antibody (ab49326) 使用論文

ab49326 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab49326.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"