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RabMAb

Anti-hHR23A 抗体 [EPR4818] (ab108592)

製品の概要

  • 製品名
    Anti-hHR23A antibody [EPR4818]
    hHR23A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4818] to hHR23A
  • アプリケーション
    適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human hHR23A.

  • ポジティブ・コントロール
    • MCF7, HeLa, Raji, and Jurkat cell lysates; Jurkat cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR4818
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能
      Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
      Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
      Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.
    • 配列類似性
      Belongs to the RAD23 family.
      Contains 2 UBA domains.
      Contains 1 ubiquitin-like domain.
    • ドメイン
      The ubiquitin-like domain mediates interaction with ATXN3.
      The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • hHR 23A antibody
      • hHR23A antibody
      • HR23A antibody
      • MGC111083 antibody
      • RAD 23a antibody
      • RAD23 homolog A (S. cerevisiae) antibody
      • RAD23 homolog A antibody
      • RAD23 yeast homolog A antibody
      • RAD23A antibody
      • RD23A_HUMAN antibody
      • UV excision repair protein RAD23 antibody
      • UV excision repair protein RAD23 homolog A antibody
      see all

    Anti-hHR23A antibody [EPR4818] 画像

    • All lanes : Anti-hHR23A antibody [EPR4818] (ab108592) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • ab108592 at 1/10 dilution staining hHR23A in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG (negative) in green.

    Anti-hHR23A antibody [EPR4818] (ab108592) 使用論文

    ab108592 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (u2os)
    Specification
    u2os
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jan 02 2014

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 02 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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