製品の概要

  • 製品名Anti-HGS (phospho Y334) antibody
    HGS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HGS (phospho Y334)
  • 特異性ab62447 detects endogenous levels of HGS only when phosphorylated at tyrosine 334.
  • アプリケーション適用あり: ICC/IF, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human HGS around the phosphorylation site of tyrosine 334 (R-N-YP-W-E).

  • ポジティブ・コントロール
    • IHC-P: Human brain tissue ICC/IF: HeLa cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab62447 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
  • 組織特異性Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
  • 配列類似性Contains 1 FYVE-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • ドメインHas a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
    The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions.
  • 翻訳後修飾Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
    Ubiquitinated by ITCH.
  • 細胞内局在Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Hepatocyte growth factor regulated tyrosine kinase substrate antibody
    • Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
    • HGF-regulated tyrosine kinase substrate antibody
    • HGNC:4897 antibody
    • HGS antibody
    • HGS_HUMAN antibody
    • Hrs antibody
    • Human growth factor regulated tyrosine kinase substrate antibody
    • Protein pp110 antibody
    • ZFYVE8 antibody
    see all

Anti-HGS (phospho Y334) antibody 画像

  • Immunohistochemistry analysis of HGS in human brain tissue (paraffin section) using ab62447 (1/50); in the presence or absence of phosphopeptide.
  • Immunofluorescence analysis of HGS in HeLa cells treated with Forskolin (40nM, 15mins); using ab62447 (1/500).

Anti-HGS (phospho Y334) antibody (ab62447) 使用論文

ab62447 has not yet been referenced specifically in any publications.

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