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RabMAb

Anti-HGFA Inhibitor 1 抗体 [EPR15652] (ab189511)

製品の概要

  • 製品名
    Anti-HGFA Inhibitor 1 antibody [EPR15652]
  • 製品の詳細
    Rabbit monoclonal [EPR15652] to HGFA Inhibitor 1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment within Human HGFA Inhibitor 1 aa 150-250. The exact sequence is proprietary.
    Database link: O43278

  • ポジティブ・コントロール
    • Human placenta, HCT116 and HeLa cell lysates; Human transitional cell bladder carcinoma tissue.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR15652
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab189511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14).
  • 配列類似性
    Contains 2 BPTI/Kunitz inhibitor domains.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MANSC domain.
  • ドメイン
    This inhibitor contains two inhibitory domains.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HAI 1 antibody
    • HAI-1 antibody
    • HAI1 antibody
    • Hepatocyte growth factor activator inhibitor type 1 antibody
    • Kunitz-type protease inhibitor 1 antibody
    • SPINT1 antibody
    • SPIT1_HUMAN antibody
    see all

画像

  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human transitional cell carcinoma of bladder sections labeling HGFA Inhibitor 1 with ab189511 at a 1/100 dilution. Secondary antibody used was a ready to use HRP polymer for rabbit IgG. Counterstained with hematoxylin.

  • Anti-HGFA Inhibitor 1 antibody [EPR15652] (ab189511) at 1/10000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 58 kDa

  • Anti-HGFA Inhibitor 1 antibody [EPR15652] (ab189511) at 1/50000 dilution + HCT116 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 58 kDa

  • Anti-HGFA Inhibitor 1 antibody [EPR15652] (ab189511) at 1/50000 dilution + Human placenta lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 58 kDa

参考文献

This product has been referenced in:
  • Sun P  et al. Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells. Oncotarget 9:12682-12694 (2018). Read more (PubMed: 29560101) »
  • Zhu L  et al. Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis. Oncogene 35:5388-5399 (2016). IHC-P ; Human . Read more (PubMed: 27109105) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH9,0
Permeabilization
Yes - Wash - Buffer from Dako with Tween
Specification
Stomach
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Oct 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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