Anti-HAI-1 抗体 [EPR15652] (ab189511)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15652] to HAI-1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HAI-1 antibody [EPR15652]
HAI-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15652] to HAI-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human placenta, HCT116 and HeLa cell lysates; Human transitional cell bladder carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15652 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab189511の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). -
配列類似性
Contains 2 BPTI/Kunitz inhibitor domains.
Contains 1 LDL-receptor class A domain.
Contains 1 MANSC domain. -
ドメイン
This inhibitor contains two inhibitory domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 6692 Human
- Omim: 605123 Human
- SwissProt: O43278 Human
- Unigene: 233950 Human
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別名
- HAI 1 antibody
- HAI antibody
- HAI-1 antibody
see all
画像
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human transitional cell carcinoma of bladder sections labeling HAI-1 with ab189511 at a 1/100 dilution. Secondary antibody used was a ready to use HRP polymer for rabbit IgG. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/10000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 58 kDa -
Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/50000 dilution + HCT116 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 58 kDa -
Anti-HAI-1 antibody [EPR15652] (ab189511) at 1/50000 dilution + Human placenta lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 58 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (6)
ab189511 は 6 報の論文で使用されています。
- Izumi A et al. Insufficiency of hepatocyte growth factor activator inhibitor-1 confers lymphatic invasion of tongue carcinoma cells. Cancer Sci 113:2179-2193 (2022). PubMed: 35332604
- Borowicz S et al. HAI-1 is an independent predictor of lung cancer mortality and is required for M1 macrophage polarization. PLoS One 16:e0252197 (2021). PubMed: 34185790
- Sun P et al. Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells. Oncotarget 9:12682-12694 (2018). PubMed: 29560101
- Ning T et al. miR-221 and miR-222 synergistically regulate hepatocyte growth factor activator inhibitor type 1 to promote cell proliferation and migration in gastric cancer. Tumour Biol 39:1010428317701636 (2017). PubMed: 28618968
- Zhu L et al. Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis. Oncogene 35:5388-5399 (2016). IHC-P ; Human . PubMed: 27109105
- Sun P et al. Decreasing the ratio of matriptase/HAI-1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer. Mol Med Rep 14:1465-74 (2016). WB ; Human . PubMed: 27356668