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Our Abpromise guarantee covers the use of ab83760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Best results are obtained with heat-mediated antigen retrieval at pH 9.0.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21121876|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Can be blocked with HGF peptide (ab189221). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration. ELISA titre using peptide based assay 1/312500.|
ab83760 staining HGF in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunohistochemistry of Human Adrenal Gland tissue at an antibody concentration of 5.0µg/ml using anti-HGF antibody (ab83760)