製品の概要
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製品名Anti-HGF antibody
HGF 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to HGF
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由来種Goat
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特異性The product shows no cross reactivity with other tested cytokines using indirect ELISA.
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アプリケーション適用あり: ELISA, WB, Neutralisingmore details
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種交差性交差種: Human
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免疫原
Recombinant, human hepatocyte growth factor (rhHGF), expressed in the insect cell line Sf 21.
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ポジティブ･コントロール
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特記事項The endotoxin level is less than 10 ng/vial (LAL method).
Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupfer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: PBS -
Concentration information loading... -
精製度Protein G purified
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特記事項（精製）The product is purified by Protein G affinity chromatography.
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一次抗体 備考Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupfer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab10679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use a concentration of 0.5 µg/ml. Use at 0.5 µg/ml, this detects 0.31ng/well of rhHGF |
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| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 83.1 kDa. Use at a concentration of 1 µg/ml, this detects rhHGF at 50 ng/lane under both reducing and non-reducing conditions. |
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| Neutralising | Use a concentration of 1 - 16 µg/ml. ab10679 was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF, which is present at five times its own EC50 (the concentration of rhHGF producing a one-half maximal bioactivity without antibody). In this bioassay, rhHGF was pre-incubated with various dilutions of the antibody for 1hr at 37 °C in a 96 well plate. Then, 4MBr-5 cells were added to each well to give a final concentration of 3 x 105 cells/ml in 0.1ml containing 100ng/ml rhHGF. This was incubated for 48hrs at 37 °C in a 5% CO2 humidified incubator and then pulsed for 4hrs with 3H-thymidine. Cells were harvested onto glass filters and the 3H-thymidine incorporation into DNA was measured. |
ターゲット情報
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機能Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
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関連疾患Deafness, autosomal recessive, 39
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配列類似性Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 3082 Human
- Omim: 142409 Human
- SwissProt: P14210 Human
- Unigene: 396530 Human
- Unigene: 561679 Human
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別名
- DFNB39 antibody
- F TCF antibody
- Fibroblast derived tumor cytotoxic factor antibody
see all
プロトコール
参考文献
This product has been referenced in:
- Chu C et al. Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells. PLoS One 11:e0148075 (2016). WB . Read more (PubMed: 26848584) »
- Ohnishi F et al. Concentration of Rat Bone Marrow Nucleated Cells Using Hypo-osmotic Hemolysis in Distilled Water. Plast Reconstr Surg Glob Open 2:e184 (2014). Read more (PubMed: 25426367) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"