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Recombinant, human hepatocyte growth factor (rhHGF), expressed in the insect cell line Sf 21.
Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupfer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
Our Abpromise guarantee covers the use of ab10679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 µg/ml.
Use at 0.5 µg/ml, this detects 0.31ng/well of rhHGF
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 83.1 kDa.
Use at a concentration of 1 µg/ml, this detects rhHGF at 50 ng/lane under both reducing and non-reducing conditions.
|Neutralising||Use a concentration of 1 - 16 µg/ml.
ab10679 was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF, which is present at five times its own EC50 (the concentration of rhHGF producing a one-half maximal bioactivity without antibody). In this bioassay, rhHGF was pre-incubated with various dilutions of the antibody for 1hr at 37 °C in a 96 well plate. Then, 4MBr-5 cells were added to each well to give a final concentration of 3 x 105 cells/ml in 0.1ml containing 100ng/ml rhHGF. This was incubated for 48hrs at 37 °C in a 5% CO2 humidified incubator and then pulsed for 4hrs with 3H-thymidine. Cells were harvested onto glass filters and the 3H-thymidine incorporation into DNA was measured.