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Recombinant human hepatocyte growth factor (rhHGF) expressed in the insect cell line Sf 21.
Hepatocyte Growth Factor, also known as Scatter Factor (SF) and Hepatopoietin A, is a pleiotropic growth factor produced by mesodermally derived cells, such as Kupffer cells/macrophages, endothelial cells, and hepatic fat storing cells. HGF stimulates hepatocytes and other epithelial and endothelial cells to various biological actions, including mitogenic, morphogenic and motogenic activity. Anti-Human HGF neutralizes the bioactivity of rhHGF.
Our Abpromise guarantee covers the use of ab10678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 - 1 µg/ml.
Use at 0.5 - 1 µg/ml to detect <1.2 ng/well of rhHGF.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 83.1 kDa.
Use at 1 - 2 µg/ml to detect rhHGF at 50 ng/lane under non-reducing conditions.
|Neutralising||Use a concentration of 0.01 - 0.5 µg/ml.
Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 100 ng/ml rhHGF was preincubated with various dilutions of the antibody for 1 hour at 22°C, then placed in a 96-well microtiter plate. 4MBr-5 cells were added to each well and incubated for 48 hours at 37°C in a 5% CO2 humidified incubator and then pulsed for the last 24 hours with 3H-thymidine. Cells were harvested onto glass filters and the 3H-thymidine incorporation into DNA was measured.