製品の概要

  • 製品名Anti-HFE antibody
    HFE 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to HFE
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human HFE protein (NP_000401.1).

  • ポジティブ・コントロール
    • Flow Cyt: HFE expressing 293 cells WB: HepG2 cell lysate and HFE transfected 293 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab88212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
Flow Cyt Use at an assay dependent concentration. ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
  • 組織特異性Expressed in all tissues tested except brain.
  • 関連疾患Defects in HFE are a cause of hemochromatosis (HFE) [MIM:235200]. A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading.
    Defects in HFE are associated with variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. VP is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease. Note=Iron overload due to HFE mutations is a precipitating or exacerbating factor in variegate porphyria.
    Defects in HFE are associated with susceptibility to microvascular complications of diabetes type 7 (MVCD7) [MIM:612635]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ221C16.10.1 antibody
    • Hemochromatosis antibody
    • Hemochromatosis protein antibody
    • Hereditary hemochromatosis protein antibody
    • Hereditary hemochromatosis protein HLA H antibody
    • HFE 1 antibody
    • HFE antibody
    • HFE_HUMAN antibody
    • HFE1 antibody
    • HH antibody
    • High Fe antibody
    • HLA H antibody
    • HLA-H antibody
    • HLAH antibody
    • MGC:150812 antibody
    • MGC10379 antibody
    • MGC103790 antibody
    • MHC class I like protein HFE antibody
    • MVCD7 antibody
    • TFQTL2 antibody
    see all

Anti-HFE antibody 画像

  • ab88212, staining HFE expressing 293 cells (green) by flow cytometry. The black trace is the staining pattern of the negative control (293 cells).
  • Anti-HFE antibody (ab88212) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • All lanes : Anti-HFE antibody (ab88212) at 1 µg/ml

    Lane 1 : HFE transfected 293 cell lysate
    Lane 2 : non ransfected 293 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 40 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-HFE antibody (ab88212) 使用論文

ab88212 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HepG2)
Specification HepG2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 06 2014

Thank you for contacting us. This product has not been tested for specificity of the different isoforms of HFE. Based on the immunogen of this antibody, the full sequence of isoform 1, a BLAST search has shown that isoforms 3, 5 and 8 have greater than...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"