製品の概要

  • 製品名Anti-HFE antibody
    HFE 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HFE
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HFE.

    (Peptide available as ab87815.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Hela nuclear; U20S; A498

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

ターゲット情報

  • 機能Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
  • 組織特異性Expressed in all tissues tested except brain.
  • 関連疾患Defects in HFE are a cause of hemochromatosis (HFE) [MIM:235200]. A disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis, and hypogonadotropic hypogonadism. Severe effects of the disease usually do not appear until after decades of progressive iron loading.
    Defects in HFE are associated with variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. VP is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease. Note=Iron overload due to HFE mutations is a precipitating or exacerbating factor in variegate porphyria.
    Defects in HFE are associated with susceptibility to microvascular complications of diabetes type 7 (MVCD7) [MIM:612635]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ221C16.10.1 antibody
    • Hemochromatosis antibody
    • Hemochromatosis protein antibody
    • Hereditary hemochromatosis protein antibody
    • Hereditary hemochromatosis protein HLA H antibody
    • HFE 1 antibody
    • HFE antibody
    • HFE_HUMAN antibody
    • HFE1 antibody
    • HH antibody
    • High Fe antibody
    • HLA H antibody
    • HLA-H antibody
    • HLAH antibody
    • MGC:150812 antibody
    • MGC10379 antibody
    • MGC103790 antibody
    • MHC class I like protein HFE antibody
    • MVCD7 antibody
    • TFQTL2 antibody
    see all

Anti-HFE antibody 画像

  • All lanes : Anti-HFE antibody (ab75401) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 24 kDa,37 kDa (possible isoform). We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

Anti-HFE antibody (ab75401) 使用論文

ab75401 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Blood film)
Specification Blood film
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"