製品の概要
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製品名Anti-HEY1 antibody
HEY1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HEY1
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由来種Rabbit
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アプリケーション適用あり: IHC-Fr, ICC/IF, WB, IHC-Pmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Guinea pig, Cat, Dog
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免疫原
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ポジティブ･コントロール
- Jurkat cells, Fetal lung lysate, Human lung or Human kidney tissue.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab22614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with Human HEY1 peptide (ab111660). 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 as second antibody. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
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機能Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
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組織特異性Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.
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配列類似性Belongs to the HEY family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 403420 Dog
- Entrez Gene: 23462 Human
- Entrez Gene: 15213 Mouse
- Entrez Gene: 155437 Rat
- Omim: 602953 Human
- SwissProt: Q9TSZ2 Dog
- SwissProt: Q9Y5J3 Human
- SwissProt: Q9WV93 Mouse
see all -
別名
- Basic helix loop helix protein OAF1 antibody
- bHLHb31 antibody
- Cardiovascular helix loop-helix factor 2 antibody
see all
画像
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Western blot - Anti-HEY1 antibody (ab22614)Predicted band size: 34 kDa -
Western blot - Anti-HEY1 antibody (ab22614)Lane 1 : Anti-HEY1 antibody (ab22614) at 5 µg/ml
Lane 2 : Anti-HEY1 antibody (ab22614) at 2.5 µg/ml
Lane 3 : Anti-HEY1 antibody (ab22614) at 1.25 µg/ml
All lanes : Cell lysate prepared from human fetal lung cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEY1 antibody (ab22614)Paraffin embedded human lung tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.
Alveolar cells are positively labelled (indicated with arrows).
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Immunocytochemistry/ Immunofluorescence - Anti-HEY1 antibody (ab22614)This image is courtesy of an anonymous Abreviewab14404 staining HEY1 in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 16 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/500) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEY1 antibody (ab22614)Paraffin embeded human kidney tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.
Epithelial cells of renal tubules are positively labelled (indicated with arrows).
プロトコール
参考文献
This product has been referenced in:
- Yin X et al. Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast-lineage cells. Int J Mol Med 41:331-339 (2018). Read more (PubMed: 29138798) »
- Cook N et al. A phase I trial of the ?-secretase inhibitor MK-0752 in combination with gemcitabine in patients with pancreatic ductal adenocarcinoma. Br J Cancer 118:793-801 (2018). IHC-P ; Human . Read more (PubMed: 29438372) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"