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To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal.
The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity.
Our Abpromise guarantee covers the use of ab78048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/200 - 1/1000.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/200 - 1/1000. Predicted molecular weight: 35 kDa.|
ab78048 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"